MHV NSP9 (Strain A59) Mouse Monoclonal Antibody [Clone ID: 2C6.H1]
Product Data | |
Clone Name | 2C6.H1 |
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Application | IF, WB |
Recommended Dilution | Western blot: 1:1,000 (Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 in the appropriate cell lysate or extract). Immunofluorescence: 1:1,000 (Vero-E6 cells were grown on glass slides followed by infection with MHVA59 strain and fixation with PBS/3%PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min). |
Reactivity | Mouse |
Antibody Host | Mouse |
Isotype | IgG2b |
Clonality | Monoclonal |
Immunogen | E. coli derived fulllength MHV-A59 nsp9 protein. |
Specificity | This antibody is directed against the MHV-A59 nsp9 protein. |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide State: Purified State: Liquid Ig fraction |
Concentration | lot specific |
Purification | Protein A chromatography |
Conjugation | Unconjugated |
Storage | Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C or below for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. |
Stability | Shelf life: one year from despatch. |
Shipping | Blue Ice |
Database Link | |
Background | The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes. |
Synonyms | Nonstructural Protein 9, Mouse Hepatitis Virus Strain A59, Murine coronavirus |
Reference Data |
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