MHV NSP9 (Strain A59) Mouse Monoclonal Antibody [Clone ID: 2C6.H1]
Specifications
| Product Data | |
| Clone Name | 2C6.H1 |
|---|---|
| Application | IF, WB |
| Recommended Dilution | Western blot: 1:1,000 (Expect a band approximately 13 kDa in size corresponding to mature MHV-A59 nsp9 in the appropriate cell lysate or extract). Immunofluorescence: 1:1,000 (Vero-E6 cells were grown on glass slides followed by infection with MHVA59 strain and fixation with PBS/3%PFA. After washing and permeabilization of the fixed cells, antibody incubation was performed in PBS/5%FCS for 30 min). |
| Reactivity | Mouse |
| Antibody Host | Mouse |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Immunogen | E. coli derived fulllength MHV-A59 nsp9 protein. |
| Specificity | This antibody is directed against the MHV-A59 nsp9 protein. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide State: Purified State: Liquid Ig fraction |
| Concentration | lot specific |
| Purification | Protein A chromatography |
| Conjugation | Unconjugated |
| Storage | Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C or below for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Database Link | |
| Background | The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes. |
| Synonyms | Nonstructural Protein 9, Mouse Hepatitis Virus Strain A59, Murine coronavirus |
| Reference Data | |
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