CD3E Mouse Monoclonal Antibody [Clone ID: PPT3]
Product Data | |
Clone Name | PPT3 |
---|---|
Application | FC, FN, IHC, IP |
Recommended Dilution | Flow Cytometry. (Ref.1) Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections). Immunoprecipitation (Ref.1) In vitro activation of T cells. (Ref.1) |
Reactivity | Porcine |
Antibody Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Specificity | This antibody recognizes Porcine CD3e (Mr. 23 kDa). The Monoclonal antibody PPT3 is Mitogenic when presented to peripheral blood mononuclear cells in immobilized form. (Ref.1) |
Buffer | 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added. State: Purified State: Liquid purified Ig fraction. |
Concentration | lot specific |
Conjugation | Unconjugated |
Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Shipping | Blue Ice |
Database Link | |
Background | CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains (designated as gamma, delta, epsilon, zeta and eta) ranging in size from 16-28 kDa. CD3 epsilon is closely associated with the T cell antigen receptor (TCR), and is present on thymocytes, CD8+ and CD4+ positive cells. The CD3 epsilon complex mediates signal transduction. It plays a major role in signaling during antigen recognition, leading to T cell activation. The TCR/CD3E complex of T lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3 epsilon is expressed on all T cells of all mouse strains. |
Synonyms | T3/Leu-4 |
Reference Data |
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