MHC Class I H-2 Kk Mouse Monoclonal Antibody [Clone ID: 16-3-22S]

SKU AM08085PU-N

MHC Class I H-2 Kk mouse monoclonal antibody, clone 16-3-22S, Purified

Product Data

• Clone Name: 16-3-22S
• Application: CT, FC, IHC
• Recommended Dilution: Flow Cytometry. (Ref.1); Immunohistochemistry (Acetone-Fixed, Frozen Sections only).; Complement-mediated Cytotoxicity. (Ref.1)
• Reactivity: Mouse
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: C3H.SW mouse splenocytes.
• Specificity: This antibody reacts with the H-2Kk MHC class I alloantigen.; Cross-reactivity with splenocytes of SJL/Hsd mice has been observed by flow cytometric analysis. (Ref.2) It does not react with other (e.g., b, d, q) haplotypes.
• Buffer: 100 mM Borate Buffered Saline, pH 8.2.; No preservatives or amine-containing buffer salts added.; State: Purified; State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Database Link:
  P04223
• Background: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta2- microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. Lymphocytes are highly positive whereas fibroblasts or neurons show only a low level of antigen.
• Synonyms: H2-K1, H2-K, H-2K(K)

Reference Data