H2-D1 Mouse Monoclonal Antibody [Clone ID: 28-14-8]
H2-D1 mouse monoclonal antibody, clone 28-14-8, Purified
|Applications||CT, FC, FN, IHC, IP|
|Recommended Dilution||Flow Cytometry: ≤ 1 µg/106 cells. (Ref.4,7,8).
Complement-mediated cytotoxicity (Ref.1,5,6).
Immunohistochemistry on Frozen Sections. (Ref.4).
|Immunogen||C3H.SW mouse splenocytes.|
|Specificity||This antibody is specific to an epitope in the α3 domain of H-2Db in the presence or absence of ß2 microglobulin. It cross reacts with the α3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells.
Customer feedback: Clone 28-14-8 does not cross-react with Human cells.
|Formulation||1.0 ml Borate Buffered Saline, pH 8.2.
No preservatives or amine-containing buffer salts added.
State: Liquid purified Ig fraction.
|Storage||Store the antibody undiluted at 2-8°C.|
|Stability||Shelf life: one year from despatch.|
|Background||The “classical” MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic α chains noncovalently associated with the invariant ß2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.|