H2-D1 Mouse Monoclonal Antibody [Clone ID: 28-14-8]

CAT#: AM08080PU-N

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H2-D1 mouse monoclonal antibody, clone 28-14-8, Purified

Conjugation: Unconjugated Biotin FITC PE



USD 398.00

3 Days*

Size
    • 500 ug

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Specifications

Product Data
Clone Name 28-14-8
Applications CT, FC, FN, IHC, IP
Recommended Dilution Flow Cytometry: ≤ 1 µg/106 cells. (Ref.4,7,8).
Immunoprecipitation (Ref.2,3).
Complement-mediated cytotoxicity (Ref.1,5,6).
Immunohistochemistry on Frozen Sections. (Ref.4).
Blocking (Ref.6).
Reactivities Mouse
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen C3H.SW mouse splenocytes.
Specificity This antibody is specific to an epitope in the α3 domain of H-2Db in the presence or absence of ß2 microglobulin. It cross reacts with the α3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes  but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells.
Customer feedback: Clone 28-14-8 does not cross-react with Human cells.
Formulation 1.0 ml Borate Buffered Saline, pH 8.2.
No preservatives or amine-containing buffer salts added.
State: Purified
State: Liquid purified Ig fraction.
Concentration lot specific
Conjugation Unconjugated
Storage Store the antibody undiluted at 2-8°C.
Stability Shelf life: one year from despatch.
Background The “classical” MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic α chains noncovalently associated with the invariant ß2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.
Synonyms H-2D(B), H2-D1
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.