H2-D1 Mouse Monoclonal Antibody [Clone ID: 28-14-8]
Specifications
| Product Data | |
| Clone Name | 28-14-8 |
|---|---|
| Application | FC |
| Recommended Dilution | Flow Cytometry: ≤ 1 µg/106 cells. |
| Reactivity | Mouse |
| Antibody Host | Mouse |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Immunogen | C3H.SW mouse splenocytes. (Ref.1,2) |
| Specificity | This antibody is specific to an epitope in the alpha 3 domain of H-2Db. It binds to the alpha 3 domain of H-2Db in the presence or absence of beta 2 microglobulin. (Ref.7,8) It cross reacts with the alpha 3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, (Ref.9-11) but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells. (Ref.12). Customer feedback: Clone 28-14-8 does not cross-react with Human cells. |
| Buffer | 1.0 ml PBS containing 0.09% Sodium Azide as preservative Label: FITC State: Liquid purified IgG fraction Label: Fluorescein Isothiocyanate Isomer 1 |
| Concentration | lot specific |
| Conjugation | FITC |
| Storage | Store undiluted at 2-8°C. This product is photosensitive and should be protected from light. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | histocompatibility 2, D region locus 1 |
| Database Link | |
| Background | The 'classical' MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-Microglobulin. (Ref.3,4) These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. (Ref.5) |
| Synonyms | H-2D(B), H2-D1 |
| Reference Data | |
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