H2-D1 Mouse Monoclonal Antibody [Clone ID: 28-14-8]

CAT#: AM08080BT-N

H2-D1 mouse monoclonal antibody, clone 28-14-8, Biotin

Conjugation: Unconjugated Biotin FITC PE

USD 421.00

3 Days*

    • 500 ug

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Product Data
Clone Name 28-14-8
Applications FC
Recommended Dilution Flow Cytometry: < / = 1 µg/10e6 cells.
Immunohistochemistry on Frozen Tissue Sections.
Reactivities Mouse
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen C3H.SW mouse splenocytes. (Ref.1,2)
Specificity This antibody is specific to an epitope in the alpha 3 domain of H-2Db. It binds to the alpha 3 domain of H-2Db in the presence or absence of beta 2 microglobulin. (Ref.7,8) It cross reacts with the alpha 3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq.
The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, (Ref.9-11) but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells. (Ref.12).
Customer feedback: Clone 28-14-8 does not cross-react with human cells.
Formulation 1.0 mL PBS containing 0.09% Sodium Azide as preservative.
Label: Biotin
State: Liquid purified Ig fraction.
Concentration lot specific
Conjugation Biotin
Storage Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background The 'classical' MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-Microglobulin. (Ref.3,4) These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. (Ref.5)
Synonyms H-2D(B), H2-D1
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.