H2-D1 Mouse Monoclonal Antibody [Clone ID: 28-14-8]

SKU AM08080BT-N

H2-D1 mouse monoclonal antibody, clone 28-14-8, Biotin

Product Data

• Clone Name: 28-14-8
• Application: FC
• Recommended Dilution: Flow Cytometry: < / = 1 µg/10e6 cells.; Immunohistochemistry on Frozen Tissue Sections.
• Reactivity: Mouse
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: C3H.SW mouse splenocytes. (Ref.1,2)
• Specificity: This antibody is specific to an epitope in the alpha 3 domain of H-2Db. It binds to the alpha 3 domain of H-2Db in the presence or absence of beta 2 microglobulin. (Ref.7,8) It cross reacts with the alpha 3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. ; The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, (Ref.9-11) but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells. (Ref.12).; Customer feedback: Clone 28-14-8 does not cross-react with human cells.
• Buffer: 1.0 mL PBS containing 0.09% Sodium Azide as preservative.; Label: Biotin; State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Biotin
• Storage: Store the antibody undiluted at 2-8°C for one month or in (aliquots) at -20°C for longer.; This product is photosensitive and should be protected from light.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: histocompatibility 2, D region locus 1
• Database Link:
  P01899
• Background: The 'classical' MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-Microglobulin. (Ref.3,4) These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. (Ref.5)
• Synonyms: H-2D(B), H2-D1

Reference Data