Cd3e Rat Monoclonal Antibody [Clone ID: C363.29B (YCD3-1)]

SKU AM08026C5-N

Cd3e rat monoclonal antibody, clone C363.29B (YCD3-1), Cy5

Product Data

• Clone Name: C363.29B (YCD3-1)
• Application: FC
• Recommended Dilution: Flow Cytometry: Identification and enumeration of CD3+ (< / = 1 µg/10e6 cells). (Ref.1,2).
• Reactivity: Mouse
• Antibody Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Specificity: This antibody is specific to CD3e (Mr. 25 kD).
• Buffer: PBS containing 0.09% Sodium Azide as preservative.; Label: Cy5; State: Liquid purified Ig fraction.; Label: Cyanine 5
• Concentration: lot specific
• Conjugation: Cy5
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; This product is photosensitive and should be protected from light.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: CD3 antigen, epsilon polypeptide
• Database Link:
  P22646
• Background: CD3e, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. (Ref.1,2); CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation.; The monoclonal antibody C363.29B recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex. (Ref.1) In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. Immobilized C363.29 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. (Ref.1,3,4); The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody. (Ref.1)
• Synonyms: T3/Leu-4

Reference Data