PKN2 Mouse Monoclonal Antibody [Clone ID: 1D1]

CAT#: AM06663SU-N

PKN2 mouse monoclonal antibody, clone 1D1, Ascites

Product Images

Western blot analysis using PRK2 mAb against human PRK2 (AA: 555-718) recombinant protein. (Expected MW is 43.9 kDa)

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  Western blot analysis using PRK2 mouse mAb against PC-12 (1), Cos7 (2), K562 (3), Jurkat (4), Hela (5), A431 (6), C6 (7), NIH/3T3 (8) and HEK293 (9) cell lysate.

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  Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)

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  Immunohistochemical analysis of paraffin-embedded prostate tissues using PRK2 mouse mAb with DAB staining.

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  Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PRK2 mouse mAb with DAB staining.

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  Flow cytometric analysis of NIH/3T3 cells using PRK2 mouse mAb (blue) and negative control (red).

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Specifications

Product Data

• Clone Name: 1D1
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  Immunohistochemistry on paraffin sections: 1/200 - 1/1000.
  Flow cytometry: 1/200 - 1/400.
  ELISA: 1/10000.
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human PRK2 expressed in E. Coli.
  
• Specificity: This antibody reacts to PRK2.
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% sodium azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 140 kDa
• Gene Name: protein kinase N2
• Database Link:
  Entrez Gene 5586 Human
  UniProt ID Q16513
• Background: Protein-kinase-C-related kinases (PRKs) are part of the lipid-regulated protein kinases (PKC) which also include liver PAK & PKN. Human PRK1 and PRK2 share structurally similar catalytic domains, but less similar N-terminal regulatory regions suggesting different regulatory domain functions. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues. Additionally, the serine-threonine kinase PRK2 can be specifically cleaved by caspase-3 (and/or caspase-3-like subfamily members) during apoptosis.
• Synonyms: PRK2, PRKCL2, Protein kinase C-like 2