Rituximab Rat Monoclonal Antibody [Clone ID: MB2 A4]

SKU AM05879PU-N

Rituximab rat monoclonal antibody, clone MB2 A4, Purified

Product Data

• Clone Name: MB2 A4
• Application: ELISA, FC
• Recommended Dilution: ELISA: 5 µg/ml as coating antibody.; Flow Cytometry: Use 10 µl of 50 µg/ml diluted antibody to label 10⁶ cells in 100 µl.
• Reactivity: Human
• Antibody Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: F(ab)2 fragment of Rituximab.; Spleen cells from immunised rats were fused with cells of the NS-1 mouse myeloma cell line
• Specificity: This antibody specifically recognises the idiotypic determinants expressed by the Rituximab humanised monoclonal antibody. It does not recognise other CD20 antibodies.; This antibody has been used in ELISA assays to monitor the levels of Rituximab in patient serum following therapy.; It has been used to detect Rituximab bound to the surface of the Raji B cell line, however detection of Rituximab bound 'in vivo' to B-CLL cells has not been demonstrated. It is possible that complement deposition on Rituximab opsonised cells inhibits binding of the anti-Rituximab antibody to cell bound Rituximab (3).
• Buffer: TRIS buffered saline pH 8.0; State: Purified; State: Liquid purified IgG fraction from Tissue Culture Supernatant; Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Background: Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen.
• Synonyms: Rituxan, MabThera

Reference Data