Rituximab Rat Monoclonal Antibody [Clone ID: MB2 A4]
$1,525.00
5 Days*
Specifications
| Product Data | |
| Clone Name | MB2 A4 |
|---|---|
| Application | ELISA, FC |
| Recommended Dilution | ELISA: 5 µg/ml as coating antibody. Flow Cytometry: Use 10 µl of 50 µg/ml diluted antibody to label 106 cells in 100 µl. |
| Reactivity | Human |
| Antibody Host | Rat |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Immunogen | F(ab)2 fragment of Rituximab. Spleen cells from immunised rats were fused with cells of the NS-1 mouse myeloma cell line |
| Specificity | This antibody specifically recognises the idiotypic determinants expressed by the Rituximab humanised monoclonal antibody. It does not recognise other CD20 antibodies. This antibody has been used in ELISA assays to monitor the levels of Rituximab in patient serum following therapy. It has been used to detect Rituximab bound to the surface of the Raji B cell line, however detection of Rituximab bound 'in vivo' to B-CLL cells has not been demonstrated. It is possible that complement deposition on Rituximab opsonised cells inhibits binding of the anti-Rituximab antibody to cell bound Rituximab (3). |
| Buffer | TRIS buffered saline pH 8.0 State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide |
| Concentration | lot specific |
| Purification | Affinity Chromatography on Protein G |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Background | Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen. |
| Synonyms | Rituxan, MabThera |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.