Hexokinase 1 (HK1) Mouse Monoclonal Antibody [Clone ID: 4D7]

SKU AM05641PU-S

Hexokinase 1 (HK1) mouse monoclonal antibody, clone 4D7, Purified

Product Data

• Clone Name: 4D7
• Application: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.; Western blot (1:1,000).; Immunofluorescence (1:1,000).; Immunohistochemistry on Paraffin Sections (5 µg/ml):; This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant Human Hexokinase 1 (1-917 aa) purified from E.coli
• Specificity: The antibody recognizes all four isoforms of Hexokinase (1~4) in recombinant protein.
• Buffer: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol; State: Purified; State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: hexokinase 1
• Database Link:
  P19367
• Background: Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase1 is found in the adipose tissue and liver and encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. A deficiency in hexokinase 1 is implicated in the rare hereditary autosomal recessive disease known as nonspherocytic haemolytic anaemia.
• Synonyms: HK1, Hexokinase type I, Brain form hexokinase

Reference Data