CD56 (NCAM1) Mouse Monoclonal Antibody [Clone ID: 123C3]

SKU AM05639PU-L

CD56 (NCAM1) mouse monoclonal antibody, clone 123C3, Purified

Product Data

• Clone Name: 123C3
• Application: ELISA, FC, IHC
• Recommended Dilution: ELISA.; Flow Cytometry: Use 10 µl of 1/10-1/50 diluted antibody to label 1x10⁶ cells in 100 µl.; Immunohistochemistry on Frozen Tissues.; Immunohistochemistry on Paraffin Sections: 1/100-1/200. ; The antibody requires antigen retrieval using heat treatment prior to staining of paraffin sections.
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Small cell lung carcinoma.; Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
• Specificity: This antibody recognizes Human Neural Cell Adhesion Molecule (NCAM), otherwise known as CD56. ; Antibodies produced by clone 123C3 recognizes the 140 and 180kDa isoforms of the CD56 protein.
• Buffer: PBS; State: Purified; State: Liquid purified IgG fraction; Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: neural cell adhesion molecule 1
• Database Link:
  P13591
• Background: The CD56 molecule is a cell surface glycoprotein which is expressed on neuroendocrine cells, natural killer cells and a subset of T cells in the peripheral blood. Three main isoforms of CD56 exist. Neurons express the largest 180kD form, while haemopoietic cells express the 140kD isoform. ; In neuronal tissues, CD56 mediates homophilic and heterophilic adhesion and is implicated in neural development. Studies suggest that CD56 is also expressed on thyroid follicular epithelium and may play a role in autoimmune disease of the thyroid. CD56 is expressed in a range of tumours including tumours of the lung, neural derived malignancies and natural killer cell lymphomas.
• Synonyms: NCAM-1, N-CAM-1, NCAM

Reference Data