CD8A Mouse Monoclonal Antibody [Clone ID: 4B11]

SKU AM05530SU-T

CD8A mouse monoclonal antibody, clone 4B11

Product Data

• Clone Name: 4B11
• Application: IHC
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 1/20-1/40.; Immunohistochemistry on paraffin sections: 1/20-1/40. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. 1mM EDTA pH 8.0 is recommended for this purpose.; Positive Control Tissue: Human Tonsil.
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Synthetic peptide derived from CD8 alpha chain sequence.
• Specificity: This antibody recognizes the Human CD8 cell surface antigen, a 32kD glycoprotein expressed by the cytotoxic/suppressor subset of T-cells and weakly by NK cells.; Clone 4B11 has been reported as being suitable for use in Western blotting. AM05530SU-N is recommended for this purpose.
• Buffer: State: Lyophilized Tissue Culture Supernatant containing 0.09% Sodium Azide as preservative.
• Reconstitution Method: Restore with 1 ml distilled water.
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at 2-8°C.; Following reconstitution store the antibody undiluted at 2-8°C for one month; or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Gene Name: CD8a molecule
• Database Link:
  P01732
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
• Synonyms: CD8 alpha chain, CD8A, MAL

Reference Data