GAL4 (Activation Domain) Mouse Monoclonal Antibody [Clone ID: 5C8]

SKU AM05397PU-N

GAL4 (Activation Domain) mouse monoclonal antibody, clone 5C8, Purified

Product Data

• Clone Name: 5C8
• Application: IP, WB
• Recommended Dilution: Western blot (5-10 µg/ml).; Immunoprecipitation.
• Reactivity: Yeast
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and Mouse myeloma cells.
• Specificity: Recognizes GAL4 derivatives containing the C-terminal activation domain.
• Buffer: Phosphate buffered saline (PBS); State: Purified; State: Liquid purified Ig fraction; Preservative: 0.08% Sodium Azide
• Concentration: lot specific
• Purification: Protein A/G Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C only. ; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Database Link:
  P04386
• Background: GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.
• Synonyms: GAL81

Reference Data