GAL4 (Activation Domain) Mouse Monoclonal Antibody [Clone ID: 5C8]
$775.00
5 Days*
Specifications
| Product Data | |
| Clone Name | 5C8 |
|---|---|
| Application | IP, WB |
| Recommended Dilution | Western blot (5-10 µg/ml). Immunoprecipitation. |
| Reactivity | Yeast |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and Mouse myeloma cells. |
| Specificity | Recognizes GAL4 derivatives containing the C-terminal activation domain. |
| Buffer | Phosphate buffered saline (PBS) State: Purified State: Liquid purified Ig fraction Preservative: 0.08% Sodium Azide |
| Concentration | lot specific |
| Purification | Protein A/G Chromatography |
| Conjugation | Unconjugated |
| Storage | Store the antibody (in aliquots) at -20°C only. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Database Link | |
| Background | GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription. |
| Synonyms | GAL81 |
| Reference Data | |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.