Cyclin E1 (CCNE1) Mouse Monoclonal Antibody [Clone ID: 168]

SKU AM05221PU-N

Cyclin E1 (CCNE1) mouse monoclonal antibody, clone 168, Purified

Product Data

• Clone Name: 168
• Application: IP, WB
• Recommended Dilution: Western Blot: A 50 kDa band corresponding to human cyclin E protein is detected.; Immunoprecipitation.
• Reactivity: Human, Monkey
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant full-length human cyclin E protein.
• Buffer: PBS containing 0.08% Sodium Azide as preservative.; State: Purified; State: Liquid (sterile filtered) purified IgG fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.; Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Shipping: Blue Ice
• Gene Name: cyclin E1
• Database Link:
  P24864
• Background: Human cyclin E was originally identified by complementation of a triple cln deletion in Saccharomyces cerevisiae. Following the identification of human cyclin E protein, researchers found that breast cancers and some other solid tumors exhibited both quantitative and qualitative alterations in cyclin E protein production. In particular, the alterations in cyclin E expression in breast cancer correlated with increasing stage and grade of the tumor. These results suggested that cyclin E might be useful as a prognostic marker. More recently, Geng et al generated a mouse strain in which the coding sequences for cyclin D1 was deleted and replaced with those of human cyclin E. Replacement of cyclin D1 with cyclin E rescued all phenotypic manifestations seen in the cyclin D1 deficient mice and restored normal development to cyclin D1-dependent tissues. Based on the results of these studies, it appears that cyclin E is the major downstream target of cyclin D1. In addition to functioning downstream of cyclin D1, recent studies with Xenopus egg extracts demonstrated that cyclin E localizes to the centrosome. In these studies CDK2-cyclin E activity was shown to be required for centrosome duplication during S phase. Taken together, these data suggest a mechanism that coordinates centrosome reproduction with cycles of DNA synthesis and mitosis.
• Synonyms: Cyclin-E1, CCNE1, CCNE

Reference Data