CD40 Mouse Monoclonal Antibody [Clone ID: HI40a]

SKU AM03135AF-N

CD40 mouse monoclonal antibody, clone HI40a, Azide Free

Product Data

• Clone Name: HI40a
• Application: FC
• Recommended Dilution: Flow Cytometry (effective for indirect immunofluorescence staining flow cytometry analysis using 0.25 μg/test).
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human CD40a
• Specificity: This antibody recognizes CD40 (BP50), a 48 kDa type I single chain transmembrane glycoprotein expressed on normal and neoplastic B cells, but not on terminally differentiated plasma cells. CD40 antigen is also present on Hodgkin's and Reed-Sternberg cells, follicular dendritic cells, some macrophages, basal epithelial cells and endothelial cells.
• Buffer: Azide free phosphate buffered saline (PBS), preserved by filter sterilization, approx. pH 7.4; State: Azide Free; State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein G chromatography (purity > 95% by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: CD40 molecule
• Database Link:
  P25942
• Background: CD40 is a costimulatory molecule of the TNF receptor superfamily and is expressed on many cell types, such as B cells, monocytes/macrophages, dendritic cells, endothelial cells, fibroblasts or vascular smooth muscle cells. Interaction of CD40 and its ligand CD154 (CD40L) is required for the generation of antibody responses to T-dependent antigens as well as for the development of germinal centers and memory B cells. In monocytes/macrophages CD40 engagement induces production of pro-inflammatory cytokines and chemokines. CD40-CD154 interactions are also critical for development of CD4 T cell-dependent effector functions. CD40 links innate and adaptive immune responses to bacterial stimuli and serves as an important regulator affecting functions of other costimulatory molecules.
• Synonyms: TNFRSF5, CDw40, Bp50

Reference Data