p53 (TP53) pSer392 Mouse Monoclonal Antibody [Clone ID: FP3.2]

SKU AM03100PU-N

p53 (TP53) pSer392 mouse monoclonal antibody, clone FP3.2, Aff - Purified

Product Data

• Clone Name: FP3.2
• Application: IHC, WB
• Recommended Dilution: Western Blotting: 1 μg/ml.; Immunohistochemistry on Paraffin Sections: 10 μg/ml. ; Staining technique: Standard ABC technique (DAB+); Pretreatment: High temperature antigen retrieval (microwave, pressure cooker) in 10 mM citrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0.; Incubation Time: 1 hour at RT or overnight at 4°C; Positive Tissue: breast carcinoma with high level of wild-type p53.
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P] corresponding to amino acids 378-393 representing the C-terminus of Human p53.
• Specificity: This p53 antibody FP3.2 [FPS392] reacts with Human p53 tumour suppressor protein phosphorylated at CKII site (Ser392).
• Buffer: PBS, pH~7.4; State: Aff - Purified; State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE); Preservative: 15 mM Sodium Azide
• Concentration: lot specific
• Purification: Protein A Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: tumor protein p53
• Database Link:
  P04637
• Background: The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.
• Synonyms: Cellular tumor antigen p53, Tumor suppressor p53, Phosphoprotein p53, NY-CO-13
• Note: Predicted Molecular Weight: 53 kDa

Reference Data