beta 2 Microglobulin (B2M) Mouse Monoclonal Antibody [Clone ID: D2E9]
Product Data | |
Clone Name | D2E9 |
---|---|
Application | ELISA, FC, IF, WB |
Recommended Dilution | ELISA: In combination with HRP conjugated anti-B2MG mAb clone D3A2 (Cat.-No AM09266HR-N), clone D2E9 can be used as capturer in Sandwich ELISA for B2MG detection. Western Blot: 1 µg/ml dilution. Detect recombinant Human B2MG antigen (100 ng/lane). Flow Cytometry. Immunofluorescence: 1/50 (20 µg/ml) dilution. |
Reactivity | Human |
Antibody Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Highly purified beta 2 microglobulin from Human urine. |
Specificity | This antibody reacts to Beta 2 Microglobulin. |
Buffer | 0.01M PBS, pH 7.2 State: Aff - Purified State: Lyophilized purified IgG fraction |
Reconstitution Method | Restore with double distillated water to a final concentration to 1.0 mg/ml. |
Concentration | 1.0 mg/ml (after reconstitution) |
Purification | Affinity Chromatography on Protein G |
Conjugation | Unconjugated |
Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Shipping | Ambient |
Gene Name | beta-2-microglobulin |
Database Link | |
Background | Human beta 2 microglobulin (B2MG) is a component of major histocompatibility complex 1 (MHC1). It is abundant at the surface of lymphocytes and exists at the surface of all cell types except red cells and platelets. B2MG is normally filtered out of blood through glomeruli, but the process is impaired in patients with renal diseases. Elevated B2MG level is implicated with lymphoma, multiple myeloma, renal diseases, viral infections and auto-immune conditions. |
Synonyms | B2M, Beta-2 microglobulin, Beta 2 microglobulin |
Reference Data |
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