SERPINB9 Mouse Monoclonal Antibody [Clone ID: 7D8]

SKU AM01199PU-N

SERPINB9 mouse monoclonal antibody, clone 7D8, Purified

Product Data

• Clone Name: 7D8
• Application: FC, IHC, WB
• Recommended Dilution: Western Blot: This antibody detects a band of ~42 kda under Reducing Conditions; Flow Cytometry: Use 10 µl of 1/10-1/100 diluted antibody to label 1x10⁶ cells in 100 µl.; Membrane permeabilisation is required for this application (The use of Leucoperm is recommended for this purpose).; Immunohistochemistry on Paraffin Sections: 1/50.; This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Recommended Positive Control: Tonsil.; Also Suitable for use in Indirect Immunofluorescence (See Ref.2 for more details).
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant PI-9 produced in P. pastoris.; Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS-1 myeloma cell line.
• Specificity: This antibody is specific for SERPINB9/PI-9 (Proteinase Inhibitor 9).
• Buffer: PBS; State: Purified; State: Liquid purified Ig fraction; Preservative: 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: serpin family B member 9
• Database Link:
  P50453
• Background: PI-9 is a 42kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virusinfected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves.
• Synonyms: PI9, PI-9, Serpin B9, Cytoplasmic antiproteinase 3, CAP-3, CAP3, Proteinase inhibitor 9

Reference Data