CD16 Mouse Monoclonal Antibody [Clone ID: G7]
Specifications
| Product Data | |
| Clone Name | G7 |
|---|---|
| Application | FC |
| Recommended Dilution | Flow cytometry: Use 10 µl of neat antibody to label 10e6 cells in 100 µl. |
| Reactivity | Porcine |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Porcine peripheral blood leucocytes. |
| Specificity | This antibody recognises the porcine homologue of human CD16 (Fc gamma RIII alpha). |
| Buffer | PBS containing 0.09% Sodium azide, 1% BSA and 5% Sucrose Label: PE State: Lyophilized purified IgG fraction Label: R. Phycoerythrin (RPE) |
| Reconstitution Method | Restore with 1.0 ml distilled water. |
| Purification | Affinity chromatography on Protein G |
| Conjugation | PE |
| Storage | Prior to and following reconstitution store the antibody at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Database Link | |
| Background | CD16 is a low affinity FC gamma receptor expressed by polymorphonuclear cells (PMN), peripheral blood lymphocytes (PBL) and monocytes in pigs. |
| Synonyms | FCGR3A, CD16A, FCG3, FCGR3, IGFR3, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2 |
| Note | Clone G7 has been reported to block PBL mediated antibody-dependent cell cytotoxicity (ADCC) and partially inhibit PMN-mediated ADCC. |
| Reference Data | |
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