Phosphotyrosine (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 2A5]

SKU AM00123BT-N

Phosphotyrosine (incl. pos. control) mouse monoclonal antibody, clone 2A5, Biotin

Product Data

• Clone Name: 2A5
• Application: ELISA, IP, WB
• Recommended Dilution: ELISA: Use at 0.1 µg/ml.; Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.; Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L.; Immunoprecipitation: Use at 1-10 µg per 10e6 pervanadate-treated A431 cells.; Included Positive Control: Phosphotyrosine MW standard.
• Reactivity: Canine, Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjugated to KLH; Epitope: ... E-pY-M ...
• Specificity: This monoconal antibody (2A5) recognizes a broad spectrum of Tyrosine phosphorylated proteins in crude cell extracts.; It tolerates a negative charge N-terminal to the Phosphotyrosine residue.
• Buffer: 2 x PBS with 0.09% Sodium Azide/PEG and Sucrose.; Label: Biotin; State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
• Conjugation: Biotin
• Storage: Aliquote and freeze in liquid nitrogen.; Antibody can be stored frozen at -80°C up to 1 year. Thaw aliquots at 37°C.; Thawed aliquots may be stored at 4°C up to 3 months.
• Shipping: Blue Ice
• Background: Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains.

Reference Data