MEK1 (MAP2K1) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10B1]

SKU AM00089PU-N

MEK1 (MAP2K1) (incl. pos. control) mouse monoclonal antibody, clone 10B1, Purified

Product Data

• Clone Name: 10B1
• Application: ELISA, WB
• Recommended Dilution: Western Blot: 1μg/ml for HRPO/ECL detection.; Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.; Positive Control: Cell lysate from untreated SW480 cells.; ELISA: 0.05 μg/ml.
• Reactivity: Canine, Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide conjugated to KLH
• Specificity: This antibody specifically recognizes the N-terminus of MEK1 at 45 kDa.
• Buffer: PBS/0.09% Na-Azide/PEG and Sucrose/50% Glycerol; State: Purified; State: Liquid purified IgG
• Concentration: lot specific
• Purification: Size exclusion chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody (aliquote in liquid nitrogen) at -80°C.; Avoid repeated freezing and thawing.; Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice
• Gene Name: mitogen-activated protein kinase kinase 1
• Database Link:
  Q02750
• Background: MEK (MAP Kinase Kinase) phosphorylates the MAP kinase on both threonine and tyrosine residues of the activation loop motif TEY. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser 218/222 in MEK1 and Ser 222/226 in MEK2). These phosphorylation sites are substrates of the Raf family of kinases.
• Synonyms: MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK1, PRKMK1, MAP kinase kinase 1

Reference Data