ERK1 (MAPK3) (pT-E-pY Motif) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 12D4]
SKU
AM00084PU-N
ERK1 (MAPK3) (pT-E-pY Motif) (incl. pos. control) mouse monoclonal antibody, clone 12D4, Purified
$620.00
2 Weeks*
Specifications
| Product Data | |
| Clone Name | 12D4 |
|---|---|
| Application | ELISA, IF, IHC, IP, WB |
| Recommended Dilution | ELISA: 0.05 µg/ml. Immunoprecipitation: 1-10 µg per 10e6 pervanadate-treated A431 or HepG2 cells. Immunocytochemistry: 1-10 µg/ml. Immunohistochemistry on Frozen Sections Immunoblotting: 0.5 µg/ml for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. Included Positive Control Provided: Cell lysate from pervanadate-treated HepG2 Description: Cell lysate from treated HepG2 cells, hepatocellular carcinoma (human) Format: Lyophilized cell lysate from HepG2 cells. Serum starved HepG2 cells were treated for 15 mn with pervanadate. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins. The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation. |
| Reactivity | Canine, Human, Mouse, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Synthetic phosphopeptide conjugated to KLH |
| Specificity | This antibody specifically interacts with the pThr - Glu - pTyr motif of activated MAP kinases 1 and 2 (erk1/2). The antibody requires phosphorylation both at the threonine and the tyrosine site and does not interact with the non-phosphorylated form of the protein. Mab MAPK-12D4 shows no crossreaction with activated SAP kinases 1 or 2. |
| Buffer | 1 ml 2 x PBS / 0.09% Sodium Azide / PEG and Sucrose State: Purified State: Lyophilized purified IgG fraction |
| Reconstitution Method | Restore with 1 ml H2O (15 min, RT). |
| Purification | Size exclusion chromatography |
| Conjugation | Unconjugated |
| Storage | Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C. Avoid repeated freezing and thawing. Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Ambient |
| Gene Name | mitogen-activated protein kinase 3 |
| Database Link | |
| Background | Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y. |
| Synonyms | MAP kinase 3, MAPK 3, ERK-1, ERT2, p44-MAPK, p44-ERK1, PRKM3 |
| Reference Data | |
| Protein Categories | Enzyme: Kinases, Growth Factors, Intracellular Proteins |
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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