GST-Tag Mouse Monoclonal Antibody [Clone ID: 4H3]

SKU AM00069PU-N

GST-Tag mouse monoclonal antibody, clone 4H3, Purified

Product Data

• Clone Name: 4H3
• Application: ELISA, IP, WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.; Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.; ELISA: 0.05 µg/ml.; Immunoprecipitation: 1-10 µg/ml.
• Reactivity: Schistosoma japonicum
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant GST (pGEX), Schistosoma japonicum
• Specificity: This antibody specifically interacts with GST of Schistosoma japonicum that is encoded by the pGEX expression vectors. The antibody does not interact with mammalian GST proteins.
• Buffer: 1 ml 2xPBS / 0.09% Sodium Azide / PEG and Sucrose; State: Purified; State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 1 ml H₂O (15 min, RT).
• Purification: Size Exclusion Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.; Avoid repeated freezing and thawing.; Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Database Link:
  P08515
• Background: The glutathione-S-transferase (GST) of Schistosoma japonicum is widely used as fusion partner in protein expression systems. The GST can be used for affinity purification of fusion proteins on immobilized glutathion as well as tag sequence if antibodies specific for the expressed protein are not available.
• Synonyms: Glutathione S-transferase Tag, GST26-Tag

Reference Data