CREB1 pSer133/Ser63 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 10E9]

SKU AM00027PU-N

CREB1 pSer133/Ser63 (incl. pos. control) mouse monoclonal antibody, clone 10E9, Purified

Product Data

• Clone Name: 10E9
• Application: ELISA, WB
• Recommended Dilution: Western Blot: 0.5 µg/ml for HRPO/ECL detection.; Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.; ELISA: 0.05 µg/ml.
• Reactivity: Canine, Human, Mouse
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic phosphopeptide conjugated to KLH.
• Specificity: This antibody specifically recognizes CREB phosphorylated at serine 133 and ATF1 phosphorylated at serine 63 in Western blot and ELISA applications.
• Buffer: 1 ml 2 x PBS / 0.09 % Na-azide / PEG and Sucrose; State: Purified; State: Lyophilized purified IgG
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Size exclusion chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.; Avoid repeated freezing and thawing.; Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Gene Name: cAMP responsive element binding protein 1
• Database Link:
  P16220
• Background: The transcription factor CREB activates transcription in response to a variety of extracellular signals. Various kinases, including Protein kinase A (PKA) , and CaM kinase II, regulate CREB activity by phosphorylation of serine 133.; Molecular Weight: CREB:43kDa; ATF1:38kDa
• Synonyms: cAMP Response Element-Binding protein, CREB1, CREB-1

Reference Data