beta Catenin (CTNNB1) pTyr86 (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 24E1]

SKU AM00015PU-N

beta Catenin (CTNNB1) pTyr86 (incl. pos. control) mouse monoclonal antibody, clone 24E1, Purified

Product Data

• Clone Name: 24E1
• Application: ELISA, WB
• Recommended Dilution: Western Blot: 1 µg/ml for HRPO/ECL detection.; Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.; ELISA: use at 0.05 µg/ml.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Phosphopeptide conjugated to hemocyanin.
• Specificity: This antibody specifically recognizes β-catenin phosphorylated at tyrosine 86 at 90 kDa.
• Buffer: 1 ml 2 x PBS / 0.09% Sodium Azide / PEG and Sucrose; State: Purified; State: Lyophilized
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Size exclusion chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.; Avoid repeated freezing and thawing.; Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Gene Name: catenin beta 1
• Database Link:
  P35222
• Background: The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The direct interaction of β-catenin with the cytoplasmic domain of cadherins plays a crucial role for cell-cell adhesion and signal transmission between neighbouring cells. Recent studies indicate that β-catenin may also play a role in tumorigenesis since it forms complexes with the tumor suppressor gene product APC. β-catenin directly interacts and constitutively activates transcription factors of the TCF/LEF gene family. Thus it is proposed that β-catenin plays a dual role not only in the maintainance and regulation of cell-cell interactions but also in the regulation of gene activity. Additionally, β-catenin is a substrate of both receptor and non-receptor tyrosine kinases. Tyrosine 86 and tyrosine 654 are substrates of EGF receptor and src family kinases while tyrosine 142 is a substrate of fer tyrosine kinase.
• Synonyms: CTNNB1, CTNNB, Beta-catenin

Reference Data