SLC18A3 / VACHT Goat Polyclonal Antibody

CAT#: 24286

SLC18A3 / VACHT goat polyclonal antibody

Product Images

IHC image of neurons in the rat basal forebrain staining for VAT. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% rabbit serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, rabbit anti-goat secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

• 

  Staining with VAT (Vesicular Acetylcholine Transporter) Antibody 24286

Specifications

Product Data

• Applications: EM, IF, IHC
• Recommended Dilution: IHC Quality Control:
  The antibody produces significant staining of VAChT at dilutions of 1/200 – 1/400 using indirect immunofluorescence and dilutions of 1/5,000–1/10,000 using biotin-avidin/HRP technique in rat basal forebrain. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Immunolabeling is completely abolished by preadsorption with synthetic rat VAT (VAChT) (511–530). Immunolabeling of transfected cells demonstrates no cross reactivity with vesicular monoamine transporters.
• Reactivities: Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: C-terminal synthetic peptide sequence corresponding to amino acids (511–530) from the cloned rat VACHT
• Formulation: State: Serum
  State: Lyophilized whole serum (100 μL)
  Preservative: ≤ 0.09% sodium azide
• Conjugation: Unconjugated
• Storage: After reconstitution, use immediately or refrigerate at 2º-8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles.
• Synonyms: VAChT, Vesicular acetylcholine transporter