IHC 101

What is IHC?

IHC is a common immunostaining method used for the detection of target protein in tissue sections. The protein can be visualized in tissue via a specific antigen-antibody reaction.

Who uses IHC?

IHC is widely used in diagnostic and research laboratories. It is a powerful tool to study the tissue distribution of an antigen in normal and cancer tissues.

How do you perform IHC?

A typical IHC experiment requires:

  • Tissue slides
  • IHC validated primary antibody
  • secondary antibody conjugated with HRP or AP
  • chromogens, and other ancillary IHC reagents
flow chart of IHC

Flow chart of IHC process

History of IHC

history of IHC