IHC 101
What is IHC?
IHC is a common immunostaining method used for the detection of target protein in tissue sections. The protein can be visualized in tissue via a specific antigen-antibody reaction.
Who uses IHC?
IHC is widely used in diagnostic and research laboratories. It is a powerful tool to study the tissue distribution of an antigen in normal and cancer tissues.
How do you perform IHC?
A typical IHC experiment requires:
- Tissue slides
- IHC validated primary antibody
- secondary antibody conjugated with HRP or AP
- chromogens, and other ancillary IHC reagents
Flow chart of IHC process
History of IHC
Chromogens and Substrates for Peroxidase
| SKU | Description | Enzyme | Specification | Datasheet | Amount |
|---|---|---|---|---|---|
| C02-12 | DAB (20x) Kit (C02-12) | 2400 | 3-component concentrated DAB, insoluble in alcohol | 12ml | |
| C02-100 | DAB Bulk (20x) Kit (C02-100) | 20000 | 3-component concentrated DAB, insoluble in alcohol | 100ml | |
| C09-12 | DAB+ 2 component Kit (C09-12) | 2400 | 2-component DAB, insoluble in alcohol | 12ml+240ml | |
| C09-100 | DAB+ 2 component Bulk Kit (C09-100) | 20000 | 2-component DAB, insoluble in alcohol | 100ml+1L |
Mounting Media for IHC with Chromogen
| SKU | Description | Suitable For | Permenant | Coverslip | Datasheet | Amount |
|---|---|---|---|---|---|---|
| E01-18 | GB-Mount: Aqueous mounting media for Chromogen - 18ml (E01-18) | AEC, AP-Red, AP-Blue | True | True | 18ml | |
| E01-100 | GB-Mount: Aqueous mounting media for Chromogen - 100ml (E01-100) | AEC, AP-Red, AP-Blue | True | True | 100ml | |
| E02-18 | O-Mount: Organic mounting media for Chromogen - 18ml (E02-18) | DAB | True | True | 18ml | |
| E03-18 | Simpo-Mount: Aqueous mounting media for Chromogen - 18ml (E03-18) | Universal | True | False | 18ml | |
| E03-100 | Simpo-Mount: Aqueous mounting media for Chromogen - 100ml (E03-100) | Universal | True | False | 100ml |
Antigen Retrieval Reagents
| SKU | Description | Specification | Datasheet | Amount |
|---|---|---|---|---|
| B04C-100 | EDTA Solution pH8.0 (20x) - 100ml (B04C-100) | For Heat Induced Epitope Retrieval (HIER), concentrated buffer | 100ml | |
| B04C-1000 | EDTA Solution pH8.0 (RTU) - 1000ml (B04C-1000) | For Heat Induced Epitope Retrieval (HIER), concentrated buffer | 1000ml | |
| B05C-1000 | Citrate Buffer pH6.0 (RTU) - 1000ml (B05C-1000) | For Heat Induced Epitope Retrieval (HIER), concentrated buffer | 1000ml | |
| B05C-100A | Citrate Buffer pH4.0 (20x) - 100ml (B05C-100A) | For Heat Induced Epitope Retrieval (HIER), concentrated buffer | 100ml | |
| B05C-100B | Citrate Buffer pH6.0 (20x) - 100ml (B05C-100B) | For Heat Induced Epitope Retrieval (HIER), concentrated buffer | 100ml | |
| B05C-100C | Citrate Buffer pH7.0 (20x) - 100ml (B05C-100C) | For Heat Induced Epitope Retrieval (HIER), concentrated buffer | 100ml | |
| E05-18 | Trypsin Solution - 18ml Trypsin + 90ml Diluent (E05-18) | For enzyme digestion of formalin fixed, paraffin embedded tissue to improve signal | 18ml+90ml | |
| E06-50 | Pepsin Solution (RTU) - 50ml (E06-50) | For enzyme digestion of formalin fixed, paraffin embedded tissue to improve signal | 50ml |
- All products are in stock and shipped directly from GBI Labs, Inc.
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