The DDK tag also known as the DYKDDDDK tag or Flag®-tag, is a polypeptide protein tag which consists of the amino acid sequence "Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys" (DYKDDDDK). It is hydrophilic in nature compared to other tags and does not denature or inactivate the proteins. It can be detected with many commercially available antibodies, the DDK sequence can be added to protein sequence to detect a protein which does not have antibodies against it. The total size of the DDK tag is 1012.9 kDa (~1KDa). It is small and can be added to the N-terminus or the C-terminus of a protein. The small size and high hydrophilicity decrease the tendency of the tag to interfere with the protein and DDK tag can be used for a variety of applications, including protein purification, protein expression, and protein localization studies. Flag is a registered trademark of Sigma-Aldrich

Immunohistology staining on AMIGO3 overexpressed cytosection TS410704P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT.

Immunohistology staining on CACNA2D1 overexpressed cytosection TS401546P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT.

PBS blank

Isotype control(10ug/ml)

DDK antibody(10ug/ml)