All round champion for DDK detection
How to select from our DDK antibodies?
|SKU||Clone ID||Host||WB||FC||IP||IF||ICC/IHC||N-term DDK tag||C-term tag||Sensitivity|
Note: We recommend TA592569 over TA50011-100.
Why is DDK important for OriGene?
Anti-DDK: One antibody to detect them all
What is DDK Tag?
The DDK tag also known as the DYKDDDDK tag or Flag®-tag, is a polypeptide protein tag which consists of the amino acid sequence "Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys" (DYKDDDDK). It is hydrophilic in nature compared to other tags and does not denature or inactivate the proteins. It can be detected with many commercially available antibodies, the DDK sequence can be added to protein sequence to detect a protein which does not have antibodies against it. The total size of the DDK tag is 1012.9 kDa (~1KDa). It is small and can be added to the N-terminus or the C-terminus of a protein. The small size and high hydrophilicity decrease the tendency of the tag to interfere with the protein and DDK tag can be used for a variety of applications, including protein purification, protein expression, and protein localization studies. Flag is a registered trademark of Sigma-Aldrich
Fig 1. A membrane protein tagged with DDK tag
Why use DDK tag?
Here are some reasons why researchers use the DYKDDDDK tag:
- Protein detection: The DDK tag can be used to detect and quantify the expression of proteins in cells or tissues. It can be detected using a commercially available antibody that recognizes the tag, allowing researchers to easily monitor the expression of their protein of interest.
- Protein purification: The DDK tag can be used as an affinity tag to purify proteins from cells or tissues. The tag can be attached to the protein of interest, and then purified using commercially available resins or columns that recognize and bind the tag.
- Protein localization: The DDK tag can be used to study the subcellular localization of proteins. By attaching the FLAG tag to a protein of interest, researchers can use fluorescence microscopy or other imaging techniques to visualize the distribution of the protein within cells or tissues.
- Minimal effect on protein function: The DDK tag is relatively small (eight amino acids) and does not tend to interfere with the normal function of the protein to which it is attached. This makes it a popular choice for researchers who want to study the properties of a protein without affecting its activity.
Validation Data for our DDK Antibody
CytoSections validation using our TA592569
Immunohistology staining on CLDN20 overexpressed cytosection TS405032P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT.
Immunohistology staining on AMIGO3 overexpressed cytosection TS410704P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT.
Immunohistology staining on CACNA2D1 overexpressed cytosection TS401546P5 with rabbit anti DDK clone OTIR5G2 C/N TA592569 at 1:2000 dilution 20m RT.
IF validation data for TA592569
Isotype control (2.5ug/ml)
Flow-cytometry validation data for TA592569