What is CRISPR CAS9?
CRISPR/Cas9, is an RNA-guided targeted genome editing tool which allows researchers to do gene knockout, knockin SNPs, insertions and deletions in cell lines and animals. The CRISPR/Cas9 genome editing system requires two components: Cas9, the endonuclease, and a guide RNA (sgRNA) which guides Cas9 to a specific location in the genome sequence. With the protospacer-adjacent motif (PAM - the sequence NGG) present at the 3′ end, Cas9 will unwind the DNA duplex and cleave both strands upon recognition of a target sequence by the guide RNA.
Products and Services
OriGene provides reagents to support a broad range of CRISPR/Cas9 gene editing applications. Click the following gene editing related product below and learn more about how we can help you.
Gene Knockout Kits
gRNA and donor cloning
CRISPRa / CRISPRi
AAVS1/ROSA26 Transgene Insertion
Functional CAS9 Protein
- Learn CRISPR from our Youtube Channel
- Webinar from Cell: Expanding CRISPR Tool Box
- Frequently Asked Questions
- HA tagging Validation Data
- CRISPR Knockout kit Manual
- CRISPR vector Manual
- CRISPR Trangene Knockin at AAVS1 and ROSA26 loci Manual
- CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) Manual
- CRISPR eBook
Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD,
Wu X, Jiang W, Marraffini LA, Zhang F.
Science 2013 Feb 15;339(6121):819-23
RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville
JE, Church GM
Science 2013 Feb 15;339(6121):823-6
|Gene Expression Resources|