Carrier Free Antibodies

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGLL (RC218358, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MGLL. Positive lysates LY402124 (100ug) and LC402124 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NTRK1 (Cat# RC213047, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTRK1(Cat# TA806413). Positive lysates LY419273 (100ug) and LC419273 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP3 (RC209597, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP3 (1:2000). Positive lysates LY416563 (100ug) and LC416563 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB1 (RC202138, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB1. Positive lysates LY410744 (100ug) and LC410744 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP3K8 (RC211171, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP3K8 (1:500). Positive lysates LY401593 (100ug) and LC401593 (20ug) can be purchased separately from OriGene.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274 (1:500). Positive lysates LY415473 (100ug) and LC415473 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min, UM800091) (1:1200)

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human IFIT1 plasmid (RC203320, lane 2), mouse IFIT1 plasmid (MR207393, lane 3) using anti-IFIT1 antibody TA500948 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of FFPE normal adjacent lung from tumor specimen using heat-induced epitope retrieval HIER at 120°C for 3min with Accel buffer pH8.7, mouse monoclonal antibody anti-ERCC1 clone 4F9 was used at 1ug/mL. Strong nuclear stain in lung pneumocytes and macrophages.

Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-FCGR1A mouse monoclonal antibody. (UM500062; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min) (1:100)

Immunohistochemical staining of paraffin-embedded human embryonic liver tissue using ALDH1L1 clone UMAB43 mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min in pressure chamber/cooker. UM500039 was diluted 1:400 and detection shown with HRP enzyme and DAB chromogen. Strong cytoplasmic and membranous staining is seen in the hepatocyte.

HEK293T cells transfected with either pCMV6-ENTRY BTK (RC211582) (Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal (TA501094), and then analyzed by flow cytometry.

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD274 (Cat# RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274(Cat# TA507087). Positive lysates LY415473 (100ug) and LC415473 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min, UM800090) (1:1200)

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human HAND1 plasmid (RC200513, lane 2), mouse HAND1 plasmid (MR202396, lane 3) using anti-HAND1 antibody TA502671 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-FOLH1mouse monoclonal antibody. (Clone UMAB25, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-SYP mouse monoclonal antibody. (UM500079; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human NPR3 plasmid (RC219453, lane 2), mouse NPR3 v2 plasmid (MR208587, lane 3), mouse NPR3 v1 plasmid (MR227616, lane 4), rat NPR3 plasmid (RR203170, lane 5) using anti-NPR3 antibody TA501021 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CD1C (Cat# RC218490, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD1C(Cat# TA505411). Positive lysates LY419762 (100ug) and LC419762 (20ug) can be purchased separately from OriGene.

Simple Western™ analysis of endogenous protein CD3E from Jurkat lysates (0.2 mg/mL) using CD3E Mouse Monoclonal Antibody #UM500048. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Anti-GOLM1 mouse monoclonal antibody (TA504112) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GOLM1 (RC200086).

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-SERPINB4mouse monoclonal antibody. (Clone UMAB16, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-SQSTM1mouse monoclonal antibody. (Clone UMAB12, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human RGS5 plasmid (RC206857, lane 2), mouse RGS5 plasmid (MR201631, lane 3) using anti-RGS5 antibody TA503075 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min, UM800104) (1:150)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ACTA2 (smooth muscle actin alpha, SMA) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800129) (1:3200)

Simple Western™ analysis of endogenous protein LMAN1 from HEK293T lysates (0.2 mg/mL) using LMAN1 Mouse Monoclonal Antibody #TA502137. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAT (RC210763, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAT. (1:5. Positive lysates LY419766 (100ug) and LC419766 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-UCHL1 (PGP9.5) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800136) (1:600)

Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human GATA3 plasmid (RC211904, hGATA3), mouse GATA3 plasmid (MR227460, mGATA3) using anti-GATA3 antibody TA809095 (1:5000@1mg/ml ). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

Immunohistochemical staining of paraffin-embedded of 3 human carcinoma of kidney using anti-CDH2 clone UMAB23 mouse monoclonal antibody at 1:400 dilution of 1.0mg/mL using Polink2 Broad HRP DAB detection kit. UM500023 requires heat-induced epitope retrieval with Citrate pH6.0 in a pressure chamber/cooker high 3min. The composit image of 3 human carcinoma of the kidney all show strong membraneous and cytoplasmic staining in the tumor cells.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-CTLA4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800141) (1:200)

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human SAMHD1 plasmid (RC206013, lane 2) using anti-SAMHD1 antibody TA502024 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Simple Western™ analysis of endogenous protein APMAP from HELA lysates (0.2 mg/mL) using APMAP Mouse Monoclonal Antibody #TA504220. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-GBA mouse monoclonal antibody. (UM800064; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MSR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, UM800139) (1:1200)

Simple Western™ analysis of endogenous protein SERPINA1 from HepG2 lysates (0.2 mg/mL) using SERPINA1 Mouse Monoclonal Antibody #TA500374. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MME (CD10) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3min, UM800127) (1:600)

Figure from citation: Positive correlation between LSD1, c-Myc, β-catenin and LGR5 expression in human colorectal tumor tissues. Expression levels of LSD1, LGR5, β-catenin and c-Myc in consecutive sections from normal colon, adenoma and CRC tumor tissues. Dilution: 1:100 View Citation. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FCGR2A mouse monoclonal antibody. (UM500051; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FOLR2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (RC210364, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1 (1:2000). Positive lysates LY401555 (100ug) and LC401555 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IDO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, UM500110) (1:600)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD80 (RC206540, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD80.

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-SDCBP mouse monoclonal antibody. (UM500057; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Mouse colon tissue using anti-beta-catenin mouse monoclonal antibody. (Clone UMAB15, dilution 1:100; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human IL6 plasmid (RC202078, hIL6), mouse IL6 plasmid (MR227281, mIL6), rat IL6 plasmid (RR201203, rIL6) using anti-IL6 antibody TA500067 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml)

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY KRT8 (Cat# RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8 (Cat# TA500021)(1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PKMYT1 (Cat# RC222657, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKMYT1(Cat# TA500874). Positive lysates LY401358 (100ug) and LC401358 (20ug) can be purchased separately from OriGene.