Tnf Mouse shRNA Plasmid (Locus ID 21926)
Tnf - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector
|Product Name||Tnf Mouse shRNA Plasmid (Locus ID 21926)|
|Synonyms||DIF; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2; Tnlg1f|
|E. coli Selection||Ampicillin|
|Kit Components||Tnf - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 21926). 5µg purified plasmid DNA per constructNon-effective 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.|
|RefSeq||BC117057, NM_001278601, NM_013693, BC137720|
|Summary||This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]|
|shRNA Design||These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact firstname.lastname@example.org. If you need a special design or shRNA sequence, please utilize our custom shRNA service.|
|Performance Guaranteed||OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at email@example.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).