Msh6 Mouse shRNA Plasmid (Locus ID 17688)

SKU TR510167

Msh6 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Product Data

• Locus ID: 17688
• Synonyms: AU044881; AW550279; GTBP; Gtmbp
• Vector: pRS
• E. coli Selection: Ampicillin
• Format: Retroviral plasmids
• Components: Msh6 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 17688). 5µg purified plasmid DNA per construct; 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
• RefSeq: BC051160, BC051634, NM_010830, NM_010830.1, NM_010830.2, BC035274
• UniProt ID: P54276
• Summary: Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction (By similarity).[UniProtKB/Swiss-Prot Function]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Shipping: Ambient