Thymidine Phosphorylase (TYMP) Human shRNA Plasmid Kit (Locus ID 1890)

CAT#: TR316523

TYMP - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector



USD 883.00

2 Weeks*

Size
    • 5 ug/vial

Product images

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Specifications

Product Data
Locus ID 1890
Synonyms ECGF; ECGF1; hPD-ECGF; MEDPS1; MNGIE; MTDPS1; PDECGF; TP
Vector pRS
E. coli Selection Ampicillin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Kit Components TYMP - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 1890). 5µg purified plasmid DNA per construct
Non-effective 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
RefSeq NM_001113755, NM_001113756, NM_001257988, NM_001257989, NM_001953, NM_001953.1, NM_001953.2, NM_001953.3, NM_001953.4, NM_001113755.1, NM_001113755.2, NM_001113756.1, NM_001113756.2, NM_001257988.1, NM_001257989.1, BC018160, BC018160.1, BC052211, BM727471, BM744178, NM_001113756.3, NM_001113755.3, NM_001953.5
UniProt ID P19971
Summary This gene encodes an angiogenic factor which promotes angiogenesis in vivo and stimulates the in vitro growth of a variety of endothelial cells. It has a highly restricted target cell specificity acting only on endothelial cells. Mutations in this gene have been associated with mitochondrial neurogastrointestinal encephalomyopathy. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Apr 2012]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.