Mir27a Mouse MicroRNA Expression Plasmid (MI0000578)

CAT#: SC400942

Expression plasmid for mouse microRNA Mir27a

Specifications

Product Data

• Species: Mouse
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  UGGCCUGAGGAGCAGGGCUUAGCUGCUUGUGAGCAAGGUCCACAGCAAAGUCGUGUUCACAGUGGCUAAGUUCCGCCCCCUGGACCC
  Mature Sequence:

  AGGGCUUAGCUGCUUGUGAGCA
  Full insert

  >MI0000578 CCTTGTGTTCAGCTATGTGAGACCCAGCCTGGTCAAGATAGGCAGGCAAGCAAGAATGCTCCAATCTCAC
  TGTCTCTTCTTTCTCTCTTTAGGTGCTACACTCCGCTCCCACCTGCTGCCCCCCTCACTGTGCCTCGGAC
  GGCTGGGGTTCCTGGGGATGGGATTTGATGCCAGTCACAAATCACATTGCCAGGGATTTCCAACTGACCC
  TGTGCTCTGCCTTGGGGGCTCCTGTCGCCAAGGATGTCTGTCTTGGGTACTGGGTAACAGAGAAGCCTAT
  CATGACAACTGGCCTGAGGAGCAGGGCTTAGCTGCTTGTGAGCAAGGTCCACAGCAAAGTCGTGTTCACA
  GTGGCTAAGTTCCGCCCCCTGGACCCCATCTCCTCAGGCCGCTGCTGCCCACCCTGCTCTGCCTCCTGGC
  TGGCAGGGCTCTGCCTCTCTCCGGGCTCCGCCTCCCGTGCCTACTGAGCTGAAACAGTTGATTCCAGTGC
  ACTGGCTCAGTTCAGCAGGAACAGGAGTCCAGCCCCCTAGGAGCTGGCAACCCTGCAGAATGTCTGCCGT
  GAGCAAGACCTGGAAACAATGGGGTAATTAGTCCAACCTAAGCCCCGTTGTCTGCTGGAGGCTCAGCCTC
  TAAGCCTGCATCTGGGTGTCCACCTTGAGATTTCTGTG
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components: 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
• Also Contains: MI0000571, MI0000578
  Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0000578
• Synonyms: Mirn27a mmu-mir-27a
• Locus ID: 387220