MirLet7a-1 Mouse MicroRNA Expression Plasmid (MI0000556)

CAT#: SC400707

Expression plasmid for mouse microRNA Let7a-1

Specifications

Product Data

• Species: Mouse
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  UUCACUGUGGGAUGAGGUAGUAGGUUGUAUAGUUUUAGGGUCACACCCACCACUGGGAGAUAACUAUACAAUCUACUGUCUUUCCUAAGGUGAU
  Mature Sequence:

  UGAGGUAGUAGGUUGUAUAGUU
  Full insert

  >MI0000556 CTAGGTTTTTGGATTGGGGACATTTAAGCTTCAGTATTAGACAGTGGTAAGAATGTAATTTCTAGATCTT
  ATAACATATTGAATTAGAGGCTTATAGCCCAGGTGTATCATATAATACAATGAAACTACAATATAGGTCA
  TATATGTAACTGTTCAGTTAAACTTCATGTTCAAGTTAAAGTAGTGAAAATCTAATATTTTCTTTTCCCT
  CATTACACAGGAAACCGGAATTACAAAGGAGAACGGCTTCCTGTGATGCTCAGCTGTGATTACTTTCAAC
  ATTCACCCTGGATGTTCTCTTCACTGTGGGATGAGGTAGTAGGTTGTATAGTTTTAGGGTCACACCCACC
  ACTGGGAGATAACTATACAATCTACTGTCTTTCCTAAGGTGATGGAAAAGTCTGCATTCATGGGGTCTCA
  TAGGAAACCAAGAACAAACTGCAGTGTTTTAAAGTATATCTTGCCTTAAAAGCATTTGCTTATGCTATGC
  ATGCTTTTTAATTTAACAAGTAGATGATGGGAATGGGCAAGTTTTTACTTACTTTGCATTCTGTGTCAAT
  GTAAATGAAGATTTAATTTTGATTGATGATGATGATTTAATGTTAACCTTTCTTGAAAGAGACTGATGCT
  TATTCCAGAAGAAAAACTTGCTCTATCAGAGTGAGGTAGT
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components: The plasmid is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Also Contains: Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0000556
• Synonyms: Mirnlet7a Mirnlet7a-1
• Locus ID: 387244