MIR215 Human MicroRNA Expression Plasmid (MI0000291)

CAT#: SC400278

Expression plasmid for human microRNA MIR215

Specifications

Product Data

• Species: Human
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Precursor:

  AUCAUUCAGAAAUGGUAUACAGGAAAAUGACCUAUGAAUUGACAGACAAUAUAGCUGAGUUUGUCUGUCAUUUCUUUAGGCCAAUAUUCUGUAUGACUGUGCUACUUCAA
  Mature Sequence:

  AUGACCUAUGAAUUGACAGAC
  Full insert

  >MI0000291 GGAATCTCCTTATATGTTTAATGGTGTTATCAAGTGTAACAGCAACTCCATGTGGACTGTGTACCAATTT
  CCAGTGGAGATGCTGTTACTTTTGATGGTTACCAACTTGCTACAATATAAAGGTATAATAAAGAAAGACA
  GAAAATTGTGGTCACTTGGACTCTCATTTGATTCCAGCAGAAAAAAAGATTTTCAGCCTGTTTTATAAAA
  TTAACAAATGATTAAGAATTAATATCAATTTTCTTAAATTCAAGTTTTGTAACACCAAAAAGATCCAATA
  ATGGAAGAGGATTAAAGTCATCATTCAGAAATGGTATACAGGAAAATGACCTATGAATTGACAGACAATA
  TAGCTGAGTTTGTCTGTCATTTCTTTAGGCCAATATTCTGTATGACTGTGCTACTTCAATATCAGAAATC
  GACTAACACCACGCAACCAACGCAATGGCAGGTACACAGAAGATAATCTGTAACACTACCATGTAAATGT
  ATTGAGTAAATAAAACATAAATATACCTAATGAAAACTTGTGTTTTTCTGACAAAGCTCATATTTATTTA
  ACCTAACTTACTGACCAGTCAACCTATGTACCATTTAATTATCAGGATGTATCAATTAAACCAGTTTGCT
  ATATTTACTAATACATACATATGATCCACCTCAGAGTCCAGAGGGCTATTCCACCAAGGAA
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Product Components: 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
• Also Contains: Both mature and minor (star) miRNA can be over expressed from the construct.

Reference Data

• RefSeq: MI0000291
• Synonyms: MIRN215 mir-215 miRNA215
• Locus ID: 406997