MIR21 Human MicroRNA Expression Plasmid (MI0000077)

CAT#: SC400271

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Expression plasmid for human microRNA MIR21



USD 396.00


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Size
    • 10 ug

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Specifications

Product Data
Species Human
Vector pCMV-MIR
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Precursor:
UGUCGGGUAGCUUAUCAGACUGAUGUUGACUGUUGAAUCUCAUGGCAACACCAGUCGAUGGGCUGUCUGACA
Mature Sequence:
UAGCUUAUCAGACUGAUGUUGA
>MI0000077 CAGTTTTCTTGCCGTTCTGTAAGTGTTTTATTCTTAGTGTGATTTTTTTCCATTGGGATGTTTTTGATTG
AACTTGTTCATTTTGTTTTGCTTGGGAGGAAAATAAACAATTTTACTTTTTTCCTTTAGGAGCATTATGA
GCATTATGTCAGAATAGAATAGAATTGGGGTTCGATCTTAACAGGCCAGAAATGCCTGGGTTTTTTTGGT
TTGTTTTTGTTTTTGTTTTTTTATCAAATCCTGCCTGACTGTCTGCTTGTTTTGCCTACCATCGTGACAT
CTCCATGGCTGTACCACCTTGTCGGGTAGCTTATCAGACTGATGTTGACTGTTGAATCTCATGGCAACAC
CAGTCGATGGGCTGTCTGACATTTTGGTATCTTTCATCTGACCATCCATATCCAATGTTCTCATTTAAAC
ATTACCCAGCATCATTGTTTATAATCAGAAACTCTGGTCCTTCTGTCTGGTGGCACTTAGAGTCTTTTGT
GCCATAATGCAGCAGTATGGAGGGAGGATTTTATGGAGAAATGGGGATAGTCTTCATGACCACAAATAAA
TAAAGGAAAACTAAGCTGCATTGTGGGTTTTGAAAAGGTTATTATACTTCTTAACAATTCTTTTTTTCAG
GGACTTTTCTAGCTGTATGAC
OTI Disclaimer All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
Product Components The plasmid is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Also Contains Both mature and minor (star) miRNA can be over expressed from the construct.
Reference Data
RefSeq MI0000077
Synonyms MIRN21 miR-21 miRNA21 hsa-mir-21
Locus ID 406991
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