mCherry Escherichia coli Recombinant Protein

CAT#: TP790040

Purified fluorescent protein mCherry, with N-terminal HIS tag, expressed in E. coli, 250ug

Product Images

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  U2AF35, U2AF65, and U2 snRNP splicing and complexing assay. Nuclear extracts were prepared from HEK293T cells expressing either mEGFP-U2B, mEGFP-SF3A3 or both mEGFP-U2AF65 and mCherry-U2AF35. The absolute and relative levels of functional labeled protein and their extent of dimerization were measured using Western blotting after affinity purification on RNA and single-molecule methods. OriGene TP790040 served as an mCherry concentration standard. Figure cited from Nucleic Acids Res, PMID: 27683217

Specifications

Product Data

• Species: Escherichia coli
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MVSKGEEDNM AIIKEFMRFK VHMEGSVNGH EFEIEGEGEG RPYEGTQTAK LKVTKGGPLP FAWDILSPQF MYGSKAYVKH PADIPDYLKL SFPEGFKWER VMNFEDGGVV TVTQDSSLQD GEFIYKVKLR GTNFPSDGPV MQKKTMGWEA SSERMYPEDG ALKGEIKQRL KLKDGGHYDA EVKTTYKAKK PVQLPGAYNV NIKLDITSHN EDYTIVEQYE RAEGRHSTGG MDELYK
• Tag: N-His
• Predicted MW: 26.5 kDa
• Concentration: >0.1 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: PBS, pH 7.4, 10% glycerol
• Bioactivity: WB standard (PMID: 27683217)
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: AHE38497