SARS-CoV-2 N Protein SARS-CoV-2 Recombinant Protein
|Expression Host||E. coli|
|Expression cDNA Clone or AA Sequence||
|Predicted MW||49.4 kDa|
|Buffer||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.|
|Reconstitution||Reconstitute with deionized water|
|Storage||Store the lyophilized protein at -20°C.
After reconstitution, store the protein at -80°C for 12 months.
Avoid repeated freezing and thawing.
|Stability||12 months from date of despatch|
|Summary||Coronavirus contain most of nucleocapsid protein. Coronavirus nucleoproteins (N proteins) localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The nucleolus is the site of ribosome biogenesis and sequesters cell cycle regulatory complexes. Two of the major components of the nucleolus are fibrillarin and nucleolin. These proteins are involved in nucleolar assembly and ribosome biogenesis and act as chaperones for the import of proteins into the nucleolus. Regarding of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is a tool for diagnostic.|
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