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FEN1 / RAD2 (1-380) Human Protein

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SKU
AR09325PU-L
Recombinant human FEN1 protein
Size:
500 ug
  $1,480.00
3 Weeks*
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Other products for "FEN1 or NM_004111"

Specifications
Specifications
Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQAAGAE QEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALVKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RILQELGLNQ EQFVDLCILL GSDYCESIRG IGPKRAVDLI QKHKSIEEIV RRLDPNKYPV PENWLHKEAH QLFLEPEVLD PESVELKWSE PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGSTKKKAKT GAAGKFKRGK
Predicted MW 42.5 kDa (380aa) confirmed by MALDI-TOF
Concentration 1mg/ml (determined by Bradford assay)
Purity > 90% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT 10% glycerol
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE
Protein Description Recombinant human FEN-1 protein was expressed in E.coli and purified by using conventional chromatography techniques.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq NP_004102.1
Locus ID 2237
UniProt ID P39748
Cytogenetics 11q12.2
Synonyms Flap structure-specific endonuclease 1, FEN-1, MF1, RAD2, Flap structure-specific endonuclease 1 DNase IV, Flap endonuclease 1, Flap structure specific endonuclease 1, hFEN1, Maturation factor 1
Summary The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. provided by RefSeq, Jul 2008
Protein Categories Intracellular Proteins
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Base excision repair, DNA replication, Non-homologous end-joining
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Documents
Resources

file-alt Citations

Novel alternative ribonucleotide excision repair pathways in human cells by DDX3X and specialized DNA polymerases
Riva, Garbelli, Casiraghi et al
Nucleic Acids Res (2020) 48 (20), 11551-11565 DOI: 10.1093/nar/gkaa948
Cited by 16
AI Summary: In this study, OriGene’s DDX3X shRNA lentiviral particles (TL313902V) and DDX3X overexpression plasmid (RC200307) were used to manipulate DDX3X expression in human cells, enabling the authors to investigate its role in alternative ribonucleotide excision repair pathways.
Snippet: herapeutic relevance in viral infections and cancer (9). It operates in all the aspects of RNA metabolism in addition to cell proliferation pathways and innate immunity (9,10). DDX3X loss promotes tumorigenesis (11), neurodegenerative diseases (12) and triggers DNA damage and embryonic lethality in mice (13). More important, recent evidence showed through chromatin immunoprecipitation analysis al
FigureFigureFigure
Efficient Recreation of t(11;22) EWSR1-FLI1(+) in Human Stem Cells Using CRISPR/Cas9
Torres-Ruiz, Martinez-Lage, Martin et al
Stem Cell Reports (2017) 8 (5), 1408-1420 DOI: 10.1016/j.stemcr.2017.04.014
AI Summary: OriGene products pCMV6-PARP1, pCMV6-MRE11, and pCMV6-FEN1 expression vectors were used to enhance DNA repair pathways in human stem cells, contributing to the study's efforts to increase the efficiency of CRISPR/Cas9-mediated t(11;22) translocation recreation.
Snippet: The altNHEJ factor expression vectors pExodus.CMV.Artemis and pExodus.CMV.Trex2 (Certo et al., 2012) were obtained from Addgene. pCMV6-PARP1, pCMV6-MRE11, and pCMV6-FEN1 were obtained from OriGene Technologies
FigureFigureFigure
Showing 1-2 of 2 papers.
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