Neuron specific enolase (1-434) Human Protein
Neuron specific enolase (1-434) human recombinant protein, 0.1 mg
|Description||Neuron specific enolase (1-434) human recombinant protein, 0.1 mg|
|Expression Host||E. coli|
|Expression cDNA Clone or AA Sequence||MSIEKIWARE ILDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DGDKQRYLGK GVLKAVDHIN STIAPALISS GLSVVEQEKL DNLMLELDGT ENKSKFGANA ILGVSLAVCK AGAAERELPL YRHIAQLAGN SDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAESFRD AMRLGAEVYH TLKGVIKDKY GKDATNVGDE GGFAPNILEN SEALELVKEA IDKAGYTEKI VIGMDVAASE FYRDGKYDLD FKSPTDPSRY ITGDQLGALY QDFVRDYPVV SIEDPFDQDD WAAWSKFTAN VGIQIVGDDL TVTNPKRIER AVEEKACNCL LLKVNQIGSV TEAIQACKLA QENGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEELGDE ARFAGHNFRN PSVL|
|Predicted MW||47 kDa|
|Purity||>95% by SDS PAGE|
|Buffer||Presentation State: Purified
State: Liquid purified protein
Buffer System: 20mM Tris pH 7.5, 100mM KCl, 5mM MgSO4
|Bioactivity||Specific: > 25,000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm
resulting from NADH at pH 6.5 at 37°C.
|Endotoxin||< 1.0 EU per 1 microgram of protein (determined by LAL method)|
|Preparation||Liquid purified protein|
|Protein Description||Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.|
|Storage||Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Summary||This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]|
|Protein Pathways||Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation|