CD11b (ITGAM) (NM_001145808) Human Untagged Clone

CAT#: SC326511

ITGAM (untagged)-Human integrin, alpha M (complement component 3 receptor 3 subunit) (ITGAM), transcript variant 1, mRNA

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD11b
• Synonyms: CD11B; CR3A; MAC-1; MAC1A; MO1A; SLEB6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001145808 edited
  TGGTTCCTCAGTGGTGCCTGCAACCCCTGGTTCACCTCCTTCCAGGTTCTGGCTCCTTCC
  AGCCATGGCTCTCAGAGTCCTTCTGTTAACAGCCTTGACCTTATGTCATGGGTTCAACTT
  GGACACTGAAAACGCAATGACCTTCCAAGAGAACGCAAGGGGCTTCGGGCAGAGCGTGGT
  CCAGCTTCAGGGATCCAGGGTGGTGGTTGGAGCCCCCCAGGAGATAGTGGCTGCCAACCA
  AAGGGGCAGCCTCTACCAGTGCGACTACAGCACAGGCTCATGCGAGCCCATCCGCCTGCA
  GGTCCCCGTGGAGGCCGTGAACATGTCCCTGGGCCTGTCCCTGGCAGCCACCACCAGCCC
  CCCTCAGCTGCTGGCCTGTGGTCCCACCGTGCACCAGACTTGCAGTGAGAACACGTATGT
  GAAAGGGCTCTGCTTCCTGTTTGGATCCAACCTACGGCAGCAGCCCCAGAAGTTCCCAGA
  GGCCCTCCGAGGGTGTCCTCAAGAGGATAGTGACATTGCCTTCTTGATTGATGGCTCTGG
  TAGCATCATCCCACATGACTTTCGGCGGATGAAGGAGTTTGTCTCAACTGTGATGGAGCA
  ATTAAAAAAGTCCAAAACCTTGTTCTCTTTGATGCAGTACTCTGAAGAATTCCGGATTCA
  CTTTACCTTCAAAGAGTTCCAGAACAACCCTAACCCAAGATCACTGGTGAAGCCAATAAC
  GCAGCTGCTTGGGCGGACACACACGGCCACGGGCATCCGCAAAGTGGTACGAGAGCTGTT
  TAACATCACCAACGGAGCCCGAAAGAATGCCTTTAAGATCCTAGTTGTCATCACGGATGG
  AGAAAAGTTTGGCGATCCCTTGGGATATGAGGATGTCATCCCTGAGGCAGACAGAGAGGG
  AGTCATTCGCTACGTCATTGGGGTGGGAGATGCCTTCCGCAGTGAGAAATCCCGCCAAGA
  GCTTAATACCATCGCATCCAAGCCGCCTCGTGATCACGTGTTCCAGGTGAATAACTTTGA
  GGCTCTGAAGACCATTCAGAACCAGCTTCGGGAGAAGATCTTTGCGATCGAGGGTACTCA
  GACAGGAAGTAGCAGCTCCTTTGAGCATGAGATGTCTCAGGAAGGCTTCAGCGCTGCCAT
  CACCTCTAATGGCCCCTTGCTGAGCACTGTGGGGAGCTATGACTGGGCTGGTGGAGTCTT
  TCTATATACATCAAAGGAGAAAAGCACCTTCATCAACATGACCAGAGTGGATTCAGACAT
  GAATGATGCTTACTTGGGTTATGCTGCCGCCATCATCTTACGGAACCGGGTGCAAAGCCT
  GGTTCTGGGGGCACCTCGATATCAGCACATCGGCCTGGTAGCGATGTTCAGGCAGAACAC
  TGGCATGTGGGAGTCCAACGCTAATGTCAAGGGCACCCAGATCGGCGCCTACTTCGGGGC
  CTCCCTCTGCTCCGTGGACGTGGACAGCAACGGCAGCACCGACCTGGTCCTCATCGGGGC
  CCCCCATTACTACGAGCAGACCCGAGGGGGCCAGGTGTCCGTGTGCCCCTTGCCCAGGGG
  GAGGGCTCGGTGGCAGTGTGATGCTGTTCTCTACGGGGAGCAGGGCCAACCCTGGGGCCG
  CTTTGGGGCAGCCCTAACAGTGCTGGGGGACGTAAATGGGGACAAGCTGACGGACGTGGC
  CATTGGGGCCCCAGGAGAGGAGGACAACCGGGGTGCTGTTTACCTGTTTCACGGAACCTC
  AGGATCTGGCATCAGCCCCTCCCATAGCCAGCGGATAGCAGGCTCCAAGCTCTCTCCCAG
  GCTCCAGTATTTTGGTCAGTCACTGAGTGGGGGCCAGGACCTCACAATGGATGGACTGGT
  AGACCTGACTGTAGGAGCCCAGGGGCACGTGCTGCTGCTCAGGTCCCAGCCAGTACTGAG
  AGTCAAGGCAATCATGGAGTTCAATCCCAGGGAAGTGGCAAGGAATGTATTTGAGTGTAA
  TGATCAGGTGGTGAAAGGCAAGGAAGCCGGAGAGGTCAGAGTCTGCCTCCATGTCCAGAA
  GAGCACACGGGATCGGCTAAGAGAAGGACAGATCCAGAGTGTTGTGACTTATGACCTGGC
  TCTGGACTCCGGCCGCCCACATTCCCGCGCCGTCTTCAATGAGACAAAGAACAGCACACG
  CAGACAGACACAGGTCTTGGGGCTGACCCAGACTTGTGAGACCCTGAAACTACAGTTGCC
  GAATTGCATCGAGGACCCAGTGAGCCCCATTGTGCTGCGCCTGAACTTCTCTCTGGTGGG
  AACGCCATTGTCTGCTTTCGGGAACCTCCGGCCAGTGCTGGCGGAGGATGCTCAGAGACT
  CTTCACAGCCTTGTTTCCCTTTGAGAAGAATTGTGGCAATGACAACATCTGCCAGGATGA
  CCTCAGCATCACCTTCAGTTTCATGAGCCTGGACTGCCTCGTGGTGGGTGGGCCCCGGGA
  GTTCAACGTGACAGTGACTGTGAGAAATGATGGTGAGGACTCCTACAGGACACAGGTCAC
  CTTCTTCTTCCCGCTTGACCTGTCCTACCGGAAGGTGTCCACGCTCCAGAACCAGCGCTC
  ACAGCGATCCTGGCGCCTGGCCTGTGAGTCTGCCTCCTCCACCGAAGTGTCTGGGGCCTT
  GAAGAGCACCAGCTGCAGCATAAACCACCCCATCTTCCCGGAAAACTCAGAGGTCACCTT
  TAATATCACGTTTGATGTAGACTCTAAGGCTTCCCTTGGAAACAAACTGCTCCTCAAGGC
  CAATGTGACCAGTGAGAACAACATGCCCAGAACCAACAAAACCGAATTCCAACTGGAGCT
  GCCGGTGAAATATGCTGTCTACATGGTGGTCACCAGCCATGGGGTCTCCACTAAATATCT
  CAACTTCACGGCCTCAGAGAATACCAGTCGGGTCATGCAGCATCAATATCAGGTCAGCAA
  CCTGGGGCAGAGGAGCCTCCCCATCAGCCTGGTGTTCTTGGTGCCCGTCCGGCTGAACCA
  GACTGTCATATGGGACCGCCCCCAGGTCACCTTCTCCGAGAACCTCTCGAGTACGTGCCA
  CACCAAGGAGCGCTTGCCCTCTCACTCCGACTTTCTGGCTGAGCTTCGGAAGGCCCCCGT
  GGTGAACTGCTCCATCGCTGTCTGCCAGAGAATCCAGTGTGACATCCCGTTCTTTGGCAT
  CCAGGAAGAATTCAATGCTACCCTCAAAGGCAACCTCTCGTTTGACTGGTACATCAAGAC
  CTCGCATAACCACCTCCTGATCGTGAGCACAGCTGAGATCTTGTTTAACGATTCCGTGTT
  CACCCTGCTGCCGGGACAGGGGGCGTTTGTGAGGTCCCAGACGGAGACCAAAGTGGAGCC
  GTTCGAGGTCCCCAACCCCCTGCCGCTCATCGTGGGCAGCTCTGTCGGGGGACTGCTGCT
  CCTGGCCCTCATCACCGCCGCGCTGTACAAGCTCGGCTTCTTCAAGCGGCAATACAAGGA
  CATGATGAGTGAAGGGGGTCCCCCGGGGGCCGAACCCCAGTAGCGGCTCCTTCCCGACAG
  AGCTGCCTCTCGGTGGCCAGCAGGACTCTGCCCAGACCACACGTAGCCCCCAGGCTGCTG
  GACACGTCGGACAGCGAAGTATCCCCGACAGGACGGGCTTGGGCT
  
• Restriction Sites: Please inquire
• ACCN: NM_001145808
• Insert Size: 3700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145808.1, NP_001139280.1
• RefSeq Size: 4745 bp
• RefSeq ORF: 3462 bp
• Locus ID: 3684
• UniProt ID: P11215
• Cytogenetics: 16p11.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Leukocyte transendothelial migration, Regulation of actin cytoskeleton
• Gene Summary: This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.