DNMT1 (NM_001130823) Human Untagged Clone

CAT#: SC325419

DNMT1 (untagged)-Human DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNMT1
• Synonyms: ADCADN; AIM; CXXC9; DNMT; HSN1E; m.HsaI; MCMT
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001130823, the custom clone sequence may differ by one or more nucleotides
  ATGCCGGCGCGTACCGCCCCAGCCCGGGTGCCCACACTGGCCGTCCCGGCCATCTCGCTG
  CCCGACGATGTCCGCAGGCGGCTCAAAGATTTGGAAAGAGACAGCTTAACAGAAAAGGAA
  TGTGTGAAGGAGAAATTGAATCTCTTGCACGAATTTCTGCAAACAGAAATAAAGAATCAG
  TTATGTGACTTGGAAACCAAATTACGTAAAGAAGAATTATCCGAGGAGGGCTACCTGGCT
  AAAGTCAAATCCCTTTTAAATAAAGATTTGTCCTTGGAGAACGGTGCTCATGCTTACAAC
  CGGGAAGTGAATGGACGTCTAGAAAACGGGAACCAAGCAAGAAGTGAAGCCCGTAGAGTG
  GGAATGGCAGATGCCAACAGCCCCCCCAAACCCCTTTCCAAACCTCGCACGCCCAGGAGG
  AGCAAGTCCGATGGAGAGGCTAAGCGTTCAAGAGACCCTCCTGCCTCAGCCTCCCAAGTA
  ACTGGGATTAGAGCTGAACCTTCACCTAGCCCCAGGATTACAAGGAAAAGCACCAGGCAA
  ACCACCATCACATCTCATTTTGCAAAGGGCCCTGCCAAACGGAAACCTCAGGAAGAGTCT
  GAAAGAGCCAAATCGGATGAGTCCATCAAGGAAGAAGACAAAGACCAGGATGAGAAGAGA
  CGTAGAGTTACATCCAGAGAACGAGTTGCTAGACCGCTTCCTGCAGAAGAACCTGAAAGA
  GCAAAATCAGGAACGCGCACTGAAAAGGAAGAAGAAAGAGATGAAAAAGAAGAAAAGAGA
  CTCCGAAGTCAAACCAAAGAACCAACACCCAAACAGAAACTGAAGGAGGAGCCGGACAGA
  GAAGCCAGGGCAGGCGTGCAGGCTGACGAGGACGAAGATGGAGACGAGAAAGATGAGAAG
  AAGCACAGAAGTCAACCCAAAGATCTAGCTGCCAAACGGAGGCCCGAAGAAAAAGAACCT
  GAAAAAGTAAATCCACAGATTTCTGATGAAAAAGACGAGGATGAAAAGGAGGAGAAGAGA
  CGCAAAACGACCCCCAAAGAACCAACGGAGAAAAAAATGGCTCGCGCCAAAACAGTCATG
  AACTCCAAGACCCACCCTCCCAAGTGCATTCAGTGCGGGCAGTACCTGGACGACCCTGAC
  CTCAAATATGGGCAGCACCCACCAGACGCGGTGGATGAGCCACAGATGCTGACAAATGAG
  AAGCTGTCCATCTTTGATGCCAACGAGTCTGGCTTTGAGAGTTATGAGGCGCTTCCCCAG
  CACAAACTGACCTGCTTCAGTGTGTACTGTAAGCACGGTCACCTGTGTCCCATCGACACC
  GGCCTCATCGAGAAGAATATCGAACTCTTCTTTTCTGGTTCAGCAAAACCAATCTATGAT
  GATGACCCATCTCTTGAAGGTGGTGTTAATGGCAAAAATCTTGGCCCCATAAATGAATGG
  TGGATCACTGGCTTTGATGGAGGTGAAAAGGCCCTCATCGGCTTCAGCACCTCATTTGCC
  GAATACATTCTGATGGATCCCAGTCCCGAGTATGCGCCCATATTTGGGCTGATGCAGGAG
  AAGATCTACATCAGCAAGATTGTGGTGGAGTTCCTGCAGAGCAATTCCGACTCGACCTAT
  GAGGACCTGATCAACAAGATCGAGACCACGGTTCCTCCTTCTGGCCTCAACTTGAACCGC
  TTCACAGAGGACTCCCTCCTGCGACACGCGCAGTTTGTGGTGGAGCAGGTGGAGAGTTAT
  GACGAGGCCGGGGACAGTGATGAGCAGCCCATCTTCCTGACACCCTGCATGCGGGACCTG
  ATCAAGCTGGCTGGGGTCACGCTGGGACAGAGGCGAGCCCAGGCGAGGCGGCAGACCATC
  AGGCATTCTACCAGGGAGAAGGACAGGGGACCCACGAAAGCCACCACCACCAAGCTGGTC
  TACCAGATCTTCGATACTTTCTTCGCAGAGCAAATTGAAAAGGATGACAGAGAAGACAAG
  GAGAACGCCTTTAAGCGCCGGCGATGTGGCGTCTGTGAGGTGTGTCAGCAGCCTGAGTGT
  GGGAAATGTAAAGCCTGCAAGGACATGGTTAAATTTGGTGGCAGTGGACGGAGCAAGCAG
  GCTTGCCAAGAGCGGAGGTGTCCCAATATGGCCATGAAGGAGGCAGATGACGATGAGGAA
  GTCGATGATAACATCCCAGAGATGCCGTCACCCAAAAAAATGCACCAGGGGAAGAAGAAG
  AAACAGAACAAGAATCGCATCTCTTGGGTCGGAGAAGCCGTCAAGACTGATGGGAAGAAG
  AGTTACTATAAGAAGGTGTGCATTGATGCGGAAACCCTGGAAGTGGGGGACTGTGTCTCT
  GTTATTCCAGATGATTCCTCAAAACCGCTGTATCTAGCAAGGGTCACGGCGCTGTGGGAG
  GACAGCAGCAACGGGCAGATGTTTCACGCCCACTGGTTCTGCGCTGGGACAGACACAGTC
  CTCGGGGCCACGTCGGACCCTCTGGAGCTGTTCTTGGTGGATGAATGTGAGGACATGCAG
  CTTTCATATATCCACAGCAAAGTGAAAGTCATCTACAAAGCCCCCTCCGAAAACTGGGCC
  ATGGAGGGAGGCATGGATCCCGAGTCCCTGCTGGAGGGGGACGACGGGAAGACCTACTTC
  TACCAGCTGTGGTATGATCAAGACTACGCGAGATTCGAGTCCCCTCCAAAAACCCAGCCA
  ACAGAGGACAACAAGTTCAAATTCTGTGTGAGCTGTGCCCGTCTGGCTGAGATGAGGCAA
  AAAGAAATCCCCAGGGTCCTGGAGCAGCTCGAGGACCTGGATAGCCGGGTCCTCTACTAC
  TCAGCCACCAAGAACGGCATCCTGTACCGAGTTGGTGATGGTGTGTACCTGCCCCCTGAG
  GCCTTCACGTTCAACATCAAGCTGTCCAGTCCCGTGAAACGCCCACGGAAGGAGCCCGTG
  GATGAGGACCTGTACCCAGAGCACTACCGGAAATACTCCGACTACATCAAAGGCAGCAAC
  CTGGATGCCCCTGAGCCCTACCGAATTGGCCGGATCAAAGAGATCTTCTGTCCCAAGAAG
  AGCAACGGCAGGCCCAATGAGACTGACATCAAAATCCGGGTCAACAAGTTCTACAGGCCT
  GAGAACACCCACAAGTCCACTCCAGCGAGCTACCACGCAGACATCAACCTGCTCTACTGG
  AGCGACGAGGAGGCCGTGGTGGACTTCAAGGCTGTGCAGGGCCGCTGCACCGTGGAGTAT
  GGGGAGGACCTGCCCGAGTGCGTCCAGGTGTACTCCATGGGCGGCCCCAACCGCTTCTAC
  TTCCTCGAGGCCTATAATGCAAAGAGCAAAAGCTTTGAAGATCCTCCCAACCATGCCCGT
  AGCCCTGGAAACAAAGGGAAGGGCAAGGGAAAAGGGAAGGGCAAGCCCAAGTCCCAAGCC
  TGTGAGCCGAGCGAGCCAGAGATAGAGATCAAGCTGCCCAAGCTGCGGACCCTGGATGTG
  TTTTCTGGCTGCGGGGGGTTGTCGGAGGGATTCCACCAAGCAGGCATCTCTGACACGCTG
  TGGGCCATCGAGATGTGGGACCCTGCGGCCCAGGCGTTCCGGCTGAACAACCCCGGCTCC
  ACAGTGTTCACAGAGGACTGCAACATCCTGCTGAAGCTGGTCATGGCTGGGGAGACCACC
  AACTCCCGCGGCCAGCGGCTGCCCCAGAAGGGAGACGTGGAGATGCTGTGCGGCGGGCCG
  CCCTGCCAGGGCTTCAGCGGCATGAACCGCTTCAATTCGCGCACCTACTCCAAGTTCAAA
  AACTCTCTGGTGGTTTCCTTCCTCAGCTACTGCGACTACTACCGGCCCCGGTTCTTCCTC
  CTGGAGAATGTCAGGAACTTTGTCTCCTTCAAGCGCTCCATGGTCCTGAAGCTCACCCTC
  CGCTGCCTGGTCCGCATGGGCTATCAGTGCACCTTCGGCGTGCTGCAGGCCGGTCAGTAC
  GGCGTGGCCCAGACTAGGAGGCGGGCCATCATCCTGGCCGCGGCCCCTGGAGAGAAGCTC
  CCTCTGTTCCCGGAGCCACTGCACGTGTTTGCTCCCCGGGCCTGCCAGCTGAGCGTGGTG
  GTGGATGACAAGAAGTTTGTGAGCAACATAACCAGGTTGAGCTCGGGTCCTTTCCGGACC
  ATCACGGTGCGAGACACGATGTCCGACCTGCCGGAGGTGCGGAATGGAGCCTCGGCACTG
  GAGATCTCCTACAACGGGGAGCCTCAGTCCTGGTTCCAGAGGCAGCTCCGGGGCGCACAG
  TACCAGCCCATCCTCAGGGACCACATCTGTAAGGACATGAGTGCATTGGTGGCTGCCCGC
  ATGCGGCACATCCCCTTGGCCCCAGGGTCAGACTGGCGCGATCTGCCCAACATCGAGGTG
  CGGCTCTCAGACGGCACCATGGCCAGGAAGCTGCGGTATACCCACCATGACAGGAAGAAC
  GGCCGCAGCAGCTCTGGGGCCCTCCGTGGGGTCTGCTCCTGCGTGGAAGCCGGCAAAGCC
  TGCGACCCCGCAGCCAGGCAGTTCAACACCCTCATCCCCTGGTGCCTGCCCCACACCGGG
  AACCGGCACAACCACTGGGCTGGCCTCTATGGAAGGCTCGAGTGGGACGGCTTCTTCAGC
  ACAACCGTCACCAACCCCGAGCCCATGGGCAAGCAGGGCCGCGTGCTCCACCCAGAGCAG
  CACCGTGTGGTGAGCGTGCGGGAGTGTGCCCGCTCCCAGGGCTTCCCTGACACCTACCGG
  CTCTTCGGCAACATCCTGGACAAGCACCGGCAGGTGGGCAATGCCGTGCCACCGCCCCTG
  GCCAAAGCCATTGGCTTGGAGATCAAGCTTTGTATGTTGGCCAAAGCCCGAGAGAGTGCC
  TCAGCTAAAATAAAGGAGGAGGAAGCTGCTAAGGAC
  
• Restriction Sites: Please inquire
• ACCN: NM_001130823
• Insert Size: 5300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_001130823.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130823.1, NP_001124295.1
• RefSeq Size: 5425 bp
• RefSeq ORF: 4899 bp
• Locus ID: 1786
• UniProt ID: P26358
• Cytogenetics: 19p13.2
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• Gene Summary: This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1, also known as Dnmt1b in PMIDs 10449766 and 10753866) represents the longest transcript and encodes the longest isoform (a).