ZAP70 (NM_001079) Human Untagged Clone

CAT#: SC323675

ZAP70 (untagged)-Kinase deficient mutant (K369M) of Human zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZAP70
• Synonyms: ADMIO2; IMD48; SRK; STCD; STD; TZK; ZAP-70
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323675 sequence for NM_001079 edited (data generated by NextGen Sequencing)
  ATGCCAGACCCCGCGGCGCACCTGCCCTTCTTCTACGGCAGCATCTCGCGTGCCGAGGCC
  GAGGAGCACCTGAAGCTGGCGGGCATGGCGGACGGGCTCTTCCTGCTGCGCCAGTGCCTG
  CGCTCGCTGGGCGGCTATGTGCTGTCGCTCGTGCACGATGTGCGCTTCCACCACTTTCCC
  ATCGAGCGCCAGCTCAACGGCACCTACGCCATTGCCGGCGGCAAAGCGCACTGTGGACCG
  GCAGAGCTCTGCGAGTTCTACTCGCGCGACCCCGACGGGCTGCCCTGCAACCTGCGCAAG
  CCGTGCAACCGGCCGTCGGGCCTCGAGCCGCAGCCGGGGGTCTTCGACTGCCTGCGAGAC
  GCCATGGTGCGTGACTACGTGCGCCAGACGTGGAAGCTGGAGGGCGAGGCCCTGGAGCAG
  GCCATCATCAGCCAGGCCCCGCAGGTGGAGAAGCTCATTGCTACGACGGCCCACGAGCGG
  ATGCCCTGGTACCACAGCAGCCTGACGCGTGAGGAGGCCGAGCGCAAACTTTACTCTGGG
  GCGCAGACCGACGGCAAGTTCCTGCTGAGGCCGCGGAAGGAGCAGGGCACATACGCCCTG
  TCCCTCATCTATGGGAAGACGGTGTACCACTACCTCATCAGCCAAGACAAGGCGGGCAAG
  TACTGCATTCCCGAGGGCACCAAGTTTGACACGCTCTGGCAGCTGGTGGAGTATCTGAAG
  CTGAAGGCGGACGGGCTCATCTACTGCCTGAAGGAGGCCTGCCCCAACAGCAGTGCCAGC
  AACGCCTCAGGGGCTGCTGCTCCCACACTCCCAGCCCACCCATCCACGTTGACTCATCCT
  CAGAGACGAATCGACACCCTCAACTCAGATGGATACACCCCTGAGCCAGCACGCATAACG
  TCCCCAGACAAACCGCGGCCGATGCCCATGGACACGAGCGTGTATGAGAGCCCCTACAGC
  GACCCAGAGGAGCTCAAGGACAAGAAGCTCTTCCTGAAGCGCGATAACCTCCTCATAGCT
  GACATTGAACTTGGCTGCGGCAACTTTGGCTCAGTGCGCCAGGGCGTGTACCGCATGCGC
  AAGAAGCAGATCGACGTGGCCATCATGGTGCTGAAGCAGGGCACGGAGAAGGCAGACACG
  GAAGAGATGATGCGCGAGGCGCAGATCATGCACCAGCTGGACAACCCCTACATCGTGCGG
  CTCATTGGCGTCTGCCAGGCCGAGGCCCTCATGCTGGTCATGGAGATGGCTGGGGGCGGG
  CCGCTGCACAAGTTCCTGGTCGGCAAGAGGGAGGAGATCCCTGTGAGCAATGTGGCCGAG
  CTGCTGCACCAGGTGTCCATGGGGATGAAGTACCTGGAGGAGAAGAACTTTGTGCACCGT
  GACCTGGCGGCCCGCAACGTCCTGCTGGTTAACCGGCACTACGCCAAGATCAGCGACTTT
  GGCCTCTCCAAAGCACTGGGTGCCGACGACAGCTACTACACTGCCCGCTCAGCAGGGAAG
  TGGCCGCTCAAGTGGTACGCACCCGAATGCATCAACTTCCGCAAGTTCTCCAGCCGCAGC
  GATGTCTGGAGCTATGGGGTCACCATGTGGGAGGCCTTGTCCTACGGCCAGAAGCCCTAC
  AAGAAGATGAAAGGGCCGGAGGTCATGGCCTTCATCGAGCAGGGCAAGCGGATGGAATGC
  CCACCAGAGTGTCCACCCGAACTGTACGCACTCATGAGTGACTGCTGGATCTACAAGTGG
  GAGGATCGCCCCGACTTCCTGACCGTGGAGCAGCGCATGCGAGCCTGTTACTACAGCCTG
  GCCAGCAAGGTGGAAGGGCCCCCAGGCAGCACACAGAAGGCTGAGGCTGCCTGTGCCTGA
  
  Clone variation with respect to NM_001079.3
  1106 a=>t;1677 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_001079 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGAGCGGCAGGGCCTC
  CCTCTCAGGCGGGGGGCTTGGGCTGCAGGGAAATCCAAACCGGCTTTGTAAGCCCCGATTCCTCACCCAG
  AACCGGCTCTCCATTGGCATTGGGACCAGAGACCCCGCAAGTGGCCTGTTTGCCTGGACATCCACCTGTA
  CGTCCCCAGGTTTCGGGAGGCCCAGGGGCGATGCCAGACCCCGCGGCGCACCTGCCCTTCTTCTAGGGCA
  GCATCTCGCGTGCCGAGGCCGAGGAGCACCTGAAGCTGGCGGGCATGGCGGACGGGCTCTTCCTGCTGCG
  CCAGTGCCTGCGCTCGCTGGGCGGCTATGTGCTGTCGCTCGTGCACGATGTGCGCTTCCACCACTTTCCC
  ATCGGAGCGCAGCTCAACGGCACCTACGCATTGCCGGCGGCAAAGCCGCACTGGTGGACCGGCAGAGAGC
  TTCTGCAAGTTCTAACTGGCCGGACCCGGACGGGCTGGCCTTGCAACTGGCCCAGGCTGTGCAACGGCGT
  CGGGCCTTAAGCGGAAACGGGGGTCTCCTCGCAACTGCCTGCAGAACGCCATGGGGCGGTGACACTACTG
  TGCCTAACTGTGAAACCTGGGAGGGCAAGGCCCTTGGGACAAGGCCTATTATACACAGGCCCGAGAGTGT
  GGGAAACTCTAGTGCTACAGGGCCTAGACCGGATTCTGTGTACAACAGACCGTAGACTTGGAGAGCCAAG
  CCAACTATACCTGGGGCAACACTGCAGATCCGTGAGAGCTCGAAGAGTACAGACCATATCCGTGCTATAT
  GGACCGGTGTACCCATATCTACTTAAAACGGTCATATCGTTCGAGACAAATGGCATCGCATCTGGTGAGC
  CTA
  
  Kinase domain sequence

  >SC323675 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TACTGGCTGCGGCACTTTGGCTCAGTGCGCCAGGGCGTGTACCGCATGCGCAAGTATGGCCGCCCCTGCC
  GTGGTGGGAGCACCGCCGCCTGGGGCAGAGGGGAGTGGCTTCACCGGGCTGTGGGACGGGAGCCGGGATG
  TCTGTCTCACAGCAGTTTGCCTGGGAAACAGACTCTGGGGCAGGACGTTGCACGCTGGAGGATTCCCTGA
  GAGAGCTCGGGACACCTGACGGGGGTGGGATGGGGCCTGGGCAGG
  
• Restriction Sites: Please inquire
• ACCN: NM_001079
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079.3, NP_001070.2
• RefSeq Size: 2450 bp
• RefSeq ORF: 1860 bp
• Locus ID: 7535
• UniProt ID: P43403
• Cytogenetics: 2q11.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Natural killer cell mediated cytotoxicity, Primary immunodeficiency, T cell receptor signaling pathway
• Gene Summary: This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).