MSK1 (RPS6KA5) (NM_004755) Human Untagged Clone

CAT#: SC323666

RPS6KA5 (untagged)-Kinase deficient mutant (K455M) of Human ribosomal protein S6 kinase, 90kDa, polypeptide 5 (RPS6KA5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MSK1
• Synonyms: MSK1; MSPK1; RLPK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004755, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGAGGAGGGTGGCAGCAGCGGCGGCGCCGCGGGGACCAGCGCGGACGGCGGCGACGGAGGAGAGC
  AGCTCCTCACTGTCAAGCACGAGCTGCGGACTGCTAATTTGACAGGACATGCTGAGAAGGTGGGAATAGA
  AAATTTTGAGCTCCTGAAGGTCCTAGGAACTGGAGCTTATGGAAAAGTATTTCTAGTTCGTAAAATAAGT
  GGCCATGATACTGGAAAGCTGTATGCCATGAAAGTTTTGAAAAAGGCAACAATCGTTCAAAAGGCCAAAA
  CCACAGAGCATACAAGGACAGAACGACAAGTCCTGGAACACATTAGGCAGTCGCCATTTTTGGTAACATT
  ACATTATGCTTTCCAGACAGAAACCAAACTTCATCTCATTTTAGATTATATAAATGGTGGTGAACTTTTT
  ACTCATCTTTCTCAAAGAGAGCGTTTCACAGAGCATGAGGTGCAGATTTATGTTGGAGAGATTGTGCTTG
  CCCTCGAACATCTCCACAAGTTGGGGATTATATATCGTGATATTAAGCTTGAGAATATTCTACTTGATTC
  TAATGGCCATGTGGTGCTGACAGATTTTGGTCTGAGTAAGGAGTTTGTGGCTGATGAAACTGAAAGAGCA
  TATTCCTTTTGTGGAACTATTGAATACATGGCACCAGATATTGTCAGAGGGGGAGATTCAGGACATGACA
  AGGCAGTTGACTGGTGGAGTTTGGGTGTTCTAATGTATGAATTACTAACTGGAGCATCTCCTTTCACTGT
  TGATGGAGAAAAAAATTCCCAAGCTGAGATATCTAGGAGAATATTAAAAAGTGAGCCTCCATATCCCCAA
  GAAATGAGTGCTTTAGCGAAAGACCTAATTCAGCGTCTTTTGATGAAAGATCCCAAGAAGAGATTGGGAT
  GTGGTCCACGTGATGCAGATGAAATCAAAGAACATCTCTTCTTTCAGAAAATAAATTGGGATGATTTAGC
  CGCCAAAAAAGTGCCTGCACCATTTAAGCCAGTCATTCGAGATGAATTAGATGTGAGTAACTTTGCAGAA
  GAGTTCACAGAAATGGATCCCACTTATTCTCCCGCAGCCCTGCCCCAGAGTTCTGAGAAGCTGTTTCAGG
  GCTATTCCTTTGTTGCTCCTTCCATCCTATTCAAGCGTAATGCAGCTGTCATAGACCCTCTTCAGTTTCA
  CATGGGAGTTGAACGTCCTGGAGTGACAAATGTTGCCAGGAGTGCAATGATGAAGGACTCTCCATTCTAT
  CAACACTATGACCTAGATTTGAAGGACAAACCCCTGGGAGAAGGTAGTTTTTCAATTTGTCGAAAGTGTG
  TGCATAAAAAAAGTAACCAAGCTTTTGCAGTCAAAATAATCAGCAAAAGGATGGAAGCCAATACTCAAAA
  GGAAATAACAGCTCTGAAACTCTGTGAAGGACACCCCAATATTGTGAAGTTGCATGAAGTTTTTCATGAT
  CAGCTTCACACGTTTCTAGTGATGGAACTTCTGAATGGAGGAGAACTGTTTGAGCGCATTAAGAAAAAGA
  AGCACTTCAGTGAGACGGAAGCCAGCTACATCATGAGGAAGCTTGTTTCAGCTGTAAGCCACATGCATGA
  TGTTGGAGTGGTGCACAGGGATCTGAAACCTGAGAATTTATTGTTCACCGATGAAAATGACAATTTGGAA
  ATTAAAATAATTGATTTTGGATTTGCACGGCTAAAGCCACCGGATAATCAGCCCCTGAAGACTCCATGCT
  TCACCCTTCATTATGCCGCCCCAGAGCTCTTGAATCAGAACGGCTACGATGAGTCCTGTGACCTGTGGAG
  CTTGGGCGTCATTTTGTACACAATGTTGTCAGGACAGGTTCCCTTCCAATCTCATGACCGAAGTTTGACG
  TGTACCAGCGCGGTGGAAATCATGAAGAAAATTAAAAAGGGAGATTTCTCCTTTGAAGGAGAAGCCTGGA
  AGAATGTATCCCAAGAGGCTAAAGATTTGATCCAAGGACTTCTCACAGTAGATCCAAACAAAAGGCTTAA
  AATGTCTGGCTTGAGGTACAATGAATGGCTACAAGATGGAAGTCAGCTGTCCTCCAATCCTCTGATGACT
  CCGGATATTCTAGGATCTTCCGGAGCTGCCGTGCATACCTGTGTGAAAGCAACCTTCCACGCCTTTAACA
  AATACAAGAGAGAGGGGTTTTGCCTTCAGAATGTTGATAAGGCCCCTTTGGCTAAGAGAAGAAAAATGAA
  AAAGACTAGCACCAGTACCGAGACGCGCAGCAGTTCCAGTGAGAGTTCCCATTCTTCTTCCTCTCATTCT
  CACGGTAAAACTACACCCACCAAGACACTGCAGCCCAGCAATCCTGCCGACAGCAATAACCCGGAGACCC
  TCTTCCAGTTCTCGGACTCAGTAGCTTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004755 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGCGGGAAAGAGACTCG
  TCTTTGCGTCCGAGTTCTGGAGCCGCCGCACCCCGACTCCTGGGGCCGCGGCAGCGGCTGCGAGGGGACG
  GGCGTCCGCTGTCTCCTGGGTTCCCCTCGTAGCGACCCGCGGGATCGGAAAAAAAGGAGAAGATGGAGGA
  GGAGGGTGGCAGCAGCGGCGGCGCCGCGGGGACCAGCGCGGACGGCGGCGACGGAGGAGAGCAGCTCCTC
  ACTGTCAAGCACGAGCTGCGGACTGCTATTTTGACAGGGACATGCTGAGAAGGTGGGAATAGAAAAATTT
  TGAGCCTCCTGAGGTCCTAGGAACTGGAGCTTATGAAAAAGTATTTCTAGTTTCGTAAAATAAGTGGCCA
  TGATACTGAAAACCTGTATGCATGAAGGTTTGAAAAAGGCACCAATGTTCCAAAGGGCCAAACCCGAACC
  ACCAGGGCCGAACGAACATCTCTGAAACATTTAGCAATGCCCATTTTGGTAACTTACATTGGCTTTCCAA
  AGAACCCAACTTCATCATTTGAGATATAAAGGGTGAACCTTACACTACTTTCAAAGAAAGCTTTCCAGAC
  ATGGTGCGATATATGGGGAAGAGTGGCTGGCTTGAACTTCCAAGTCGGAATTAATCGGATTAGCTGGAAA
  TTCTGGGACTATGCAGCGTCGCACATTCCCCTAAAGAATGGCTGAACTGAGACTCTAGTACTGGATCGCA
  AAATTCAAGG
  
  Kinase domain sequence

  >SC323666 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  ATTGATGMAACCCCTGGGAGAGGTAGTTTTTCATTTGTCGAAAGTGTGTGCATAAAAAAAGTAACCAAGC
  TTTTGCAGTCATGATAATCAGCAAAAGGATGGAAGCCAATACTCAAAAGGAAATAACAGCTCTGAAACTC
  TGTGAAGGACACCCCAATATTGTGAAGTTGCATGAAGTTTTTCATGATCAGCTTCACACGTTTCTAGTGA
  TGGAACTTCTGAATGGAGGAGAACTGTTTGAGCGCATTAAGAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_004755
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004755.2, NP_004746.2
• RefSeq Size: 3883 bp
• RefSeq ORF: 2409 bp
• Locus ID: 9252
• UniProt ID: O75582
• Cytogenetics: 14q32.11
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Bladder cancer, MAPK signaling pathway, Neurotrophin signaling pathway
• Gene Summary: Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes (PubMed:11909979, PubMed:12569367, PubMed:12763138, PubMed:9687510, PubMed:18511904, PubMed:9873047). Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin (PubMed:11909979, PubMed:9873047). Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression (PubMed:12628924, PubMed:18511904). In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress (PubMed:12628924). In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential (PubMed:12763138). Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation (PubMed:12569367). Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A (PubMed:15010469). Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN (PubMed:12773393). May also phosphorylate 'Ser-28' of histone H3 (PubMed:12773393). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14) (PubMed:12773393). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines (By similarity). Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors (By similarity). Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury (By similarity). Phosphorylates TRIM7 at 'Ser-107' in response to growth factor signaling via the MEK/ERK pathway, thereby stimulating its ubiquitin ligase activity (PubMed:25851810).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.