PERK (EIF2AK3) (NM_004836) Human Untagged Clone

SKU SC323503

EIF2AK3 (untagged)-Kinase deficient mutant (K622M) of Human eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PERK
• Synonyms: PEK; PERK; WRS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323503 sequence for NM_004836 edited (data generated by NextGen Sequencing); ATGGAGCGCGCCATCAGCCCGGGGCTGCTGGTACGGGCGCTGCTGCTGCTGCTGCTGCTG; CTGGGGCTCGCGGCAAGGACGGTGGCCGCGGGGCGCGCCCGTGGCCTCCCAGCGCCGACG; GCGGAGGCGGCGTTCGGCCTCGGGGCGGCCGCTGCTCCCACCTCAGCGACGCGAGTACCG; GCGGCGGGCGCCGTGGCTGCGGCCGAGGTGACTGTGGAGGACGCTGAGGCGCTGCCGGCA; GCCGCGGGAGAGCAGGAGCCTCGGGGTCCGGAACCAGACGATGAGACAGAGTTGCGACCG; CGCGGCAGGTCATTAGTAATTATCAGCACTTTAGATGGGAGAATTGCTGCCTTGGATCCT; GAAAATCATGGTAAAAAGCAGTGGGATTTGGATGTGGGATCCGGTTCCTTGGTGTCATCC; AGCCTTAGCAAACCAGAGGTATTTGGGAATAAGATGATCATTCCTTCCCTGGATGGAGCC; CTCTTCCAGTGGGACCGAGACCGTGAAAGCATGGAAACAGTTCCTTTCACAGTTGAATCA; CTTCTTGAATCTTCTTATAAATTTGGAGATGATGTTGTTTTGGTTGGAGGAAAATCTCTG; ACTACATATGGACTCAGTGCATATAGTGGAAAGGTGAGGTATATCTGTTCAGCTCTGGGT; TGTCGCCAATGGGATAGTGACGAAATGGAACAAGAGGAAGACATCCTGCTTCTACAGCGT; ACCCAAAAAACTGTTAGAGCTGTCGGACCTCGCAGTGGCAATGAGAAGTGGAATTTCAGT; GTTGGCCACTTTGAACTTCGGTATATTCCAGACATGGAAACGAGAGCCGGATTTATTGAA; AGCACCTTTAAGCCCAATGAGAACACAGAAGAGTCTAAAATTATTTCAGATGTGGAAGAA; CAGGAAGCTGCCATAATGGACATAGTGATAAAGGTTTCGGTTGCTGACTGGAAAGTTATG; GCATTCAGTAAGAAGGGAGGACATCTGGAATGGGAGTACCAGTTTTGTACTCCAATTGCA; TCTGCCTGGTTACTTAAGGATGGGAAAGTCATTCCCATCAGTCTTTTTGATGATACAAGT; TATACATCTAATGATGATGTTTTAGAAGATGAAGAAGACATTGTAGAAGCTGCCAGAGGA; GCCACAGAAAACAGTGTTTACTTGGGAATGTATAGAGGCCAGCTGTATCTGCAGTCATCA; GTCAGAATTTCAGAAAAGTTTCCTTCAAGTCCCAAGGCTTTGGAATCTGTCACTAATGAA; AACGCAATTATTCCTTTACCAACAATCAAATGGAAACCCTTAATTCATTCTCCTTCCAGA; ACTCCTGTCTTGGTAGGATCTGATGAATTTGACAAATGTCTCAGTAATGATAAGTTTTCT; CATGAAGAATATAGTAATGGTGCACTTTCAATCTTGCAGTATCCATATGATAATGGTTAT; TATCTACCATACTACAAGAGGGAGAGGAACAAACGAAGCACACAGATTACAGTCAGATTC; CTCGACAACCCACATTACAACAAGAATATCCGCAAAAAGGATCCTGTTCTTCTTTTACAC; TGGTGGAAAGAAATAGTTGCAACGATTTTGTTTTGTATCATAGCAACAACGTTTATTGTG; CGCAGGCTTTTCCATCCTCATCCTCACAGGCAAAGGAAGGAGTCTGAAACTCAGTGTCAA; ACTGAAAATAAATATGATTCTGTAAGTGGTGAAGCCAATGACAGTAGCTGGAATGACATA; AAAAACTCTGGATATATATCACGATATCTAACTGATTTTGAGCCAATTCAATGCCTGGGA; CGTGGTGGCTTTGGAGTTGTTTTTGAAGCTAAAAACAAAGTAGATGACTGCAATTATGCT; ATCATGAGGATCCGTCTCCCCAATAGGGAATTGGCTCGGGAAAAGGTAATGCGAGAAGTT; AAAGCCTTAGCCAAGCTTGAACACCCGGGCATTGTTAGATATTTCAATGCCTGGCTCGAA; GCACCACCAGAGAAGTGGCAAGAAAAGATGGATGAAATTTGGCTGAAAGATGAAAGCACA; GACTGGCCACTCAGCTCTCCTAGCCCAATGGATGCACCATCAGTTAAAATACGCAGAATG; GATCCTTTCTCTACAAAAGAACATATTGAAATCATAGCTCCTTCACCACAAAGAAGCAGG; TCTTTTTCAGTAGGGATTTCCTGTGACCAGACAAGTTCATCTGAGAGCCAGTTCTCACCA; CTGGAATTCTCAGGAATGGACCATGAGGACATCAGTGAGTCAGTGGATGCAGCATACAAC; CTCCAGGACAGTTGCCTTACAGACTGTGATGTGGAAGATGGGACTATGGATGGCAATGAT; GAGGGGCACTCCTTTGAACTTTGTCCTTCTGAAGCTTCTCCTTATGTAAGGTCAAGGGAG; AGAACCTCCTCTTCAATAGTATTTGAAGATTCTGGCTGTGATAATGCTTCCAGTAAAGAA; GAGCCGAAAACTAATCGATTGCATATTGGCAACCATTGTGCTAATAAACTAACTGCTTTC; AAGCCCACCAGTAGCAAATCTTCTTCTGAAGCTACATTGTCTATTTCTCCTCCAAGACCA; ACCACTTTAAGTTTAGATCTCACTAAAAACACCACAGAAAAACTCCAGCCCAGTTCACCA; AAGGTGTATCTTTACATTCAAATGCAGCTGTGCAGAAAAGAAAACCTCAAAGACTGGATG; AATGGACGATGTACCATAGAGGAGAGAGAGAGGAGCGTGTGTCTGCACATCTTCCTGCAG; ATCGCAGAGGCAGTGGAGTTTCTTCACAGTAAAGGACTGATGCACAGGGACCTCAAGCCA; TCCAACATATTCTTTACAATGGATGATGTGGTCAAGGTTGGAGACTTTGGGTTAGTGACT; GCAATGGACCAGGATGAGGAAGAGCAGACGGTTCTGACCCCAATGCCAGCTTATGCCAGA; CACACAGGACAAGTAGGGACCAAACTGTATATGAGCCCAGAGCAGATTCATGGAAACAGC; TATTCTCATAAAGTGGACATCTTTTCTTTAGGCCTGATTCTATTTGAATTGCTGTATCCA; TTCAGCACTCAGATGGAGAGAGTCAGGACCTTAACTGATGTAAGAAATCTCAAATTTCCA; CCATTATTTACTCAGAAATATCCTTGTGAGTACGTGATGGTTCAAGACATGCTCTCTCCA; TCCCCCATGGAACGACCTGAAGCTATAAACATCATTGAAAATGCTGTATTTGAGGACTTG; GACTTTCCAGGAAAAACAGTGCTCAGACAGAGGTCTCGCTCCTTGAGTTCATCGGGAACA; AAACATTCAAGACAGTCCAACAACTCCCATAGCCCTTTGCCAAGCAATTAG; ; Clone variation with respect to NM_004836.5; 497 a=>g;1865 a=>t;2110 g=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004836 unedited; ACCGCCCGTTTGAGCAACTGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAG; TGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGGCTGAG; ACGTGGCCAGGGGAACACGGCTGGCTGTCCAGGCCGTCGGGGCGGCAGTAGGGTCCCTAGCACGTCCTTG; CCTTCTTGGGAGCTCCAAGCGGCGGGAGAGGCAGGCGTCAGTGGCTGCGCCTCCATGCCTGCGCGCGGGG; CGGGACGCTGATGGAGCGCGCCATCAGCCCGGGGCTGCTGGTACGGGCGCTGCTGCTGCTGCTGCTGCTG; CTGGGGCTCGCGGCAAGGACGGTGGCCCGCGGGGCGCGCCCGTGGCCCTCCCAGCGCCGACCGGCGGAAG; GCGGCGTTTCGGCCCTCGGGGCCGGCCGCTGCTTCCACCTCAGCGACGCGAGTCCCGGCGGCCGGGCGCC; GGGGCTGCGCCGAGGTGACTTGGGAGAACCGAGGCCTTCCGGAACGGGGGAAACCAGGACCTCGGGTTCG;
  Kinase Domain Sequence

  >SC323503 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation; AWTMCGATATCTACTGATTTTGAGCCATTCAATGCCTGGGACGTGGTGGCTTTGGAGTTGTTTTTGAAGC; TAAAAACAAAGTAGATGACTGCAATTATGCTATCATGAGGATCCGTCTCCCCAATAGGGAATTGGCTCGG; GAAAAGGTAATGCGAGAAGTTAAAGCCTTAGCCAAGCTTGAACACCCGGGCATTGTTAGATATTTCAATG; CCTGGCTCGAAGCACCACCAGAGAAGTGGCAAGAAAAGATGGATG;
• Restriction Sites: Please inquire
• ACCN: NM_004836
• Insert Size: 4280 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004836.3, NP_004827.3
• RefSeq Size: 4511 bp
• RefSeq ORF: 3351 bp
• Locus ID: 9451
• UniProt ID: Q9NZJ5
• Cytogenetics: 2p11.2
• Domains: pkinase, PQQ, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase, Secreted Protein, Transmembrane
• Protein Pathways: Alzheimer's disease
• Summary: The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.