MARK3 (NM_002376) Human Untagged Clone

CAT#: SC323372

MARK3 (untagged)-Kinase deficient mutant (K85M) of Human MAP/microtubule affinity-regulating kinase 3 (MARK3), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MARK3
• Synonyms: CTAK1; KP78; Par-1a; PAR1A; VIPB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002376, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCCACTAGGACCCCATTGCCAACGGTGAATGAACGAGACACTGAAAACCACACGTCACATGGAGATG
  GGCGTCAAGAAGTTACCTCTCGTACCAGCCGCTCAGGAGCTCGGTGTAGAAACTCTATAGCCTCCTGTGC
  AGATGAACAACCTCACATCGGAAACTACAGACTGTTGAAAACAATCGGCAAGGGGAATTTTGCAAAAGTA
  AAATTGGCAAGACATATCCTTACAGGCAGAGAGGTTGCAATAAAAATAATTGACAAAACTCAGTTGAATC
  CAACAAGTCTACAAAAGCTCTTCAGAGAAGTAAGAATAATGAAGATTTTAAATCATCCCAATATAGTGAA
  GTTATTCGAAGTCATTGAAACTGAAAAAACACTCTACCTAATCATGGAATATGCAAGTGGAGGTGAAGTA
  TTTGACTATTTGGTTGCACATGGCAGGATGAAGGAAAAAGAAGCAAGATCTAAATTTAGACAGATTGTGT
  CTGCAGTTCAATACTGCCATCAGAAACGGATCGTACATCGAGACCTCAAGGCTGAAAATCTATTGTTAGA
  TGCCGATATGAACATTAAAATAGCAGATTTCGGTTTTAGCAATGAATTTACTGTTGGCGGTAAACTCGAC
  ACGTTTTGTGGCAGTCCTCCATACGCAGCACCTGAGCTCTTCCAGGGCAAGAAATATGACGGGCCAGAAG
  TGGATGTGTGGAGTCTGGGGGTCATTTTATACACACTAGTCAGTGGCTCACTTCCCTTTGATGGGCAAAA
  CCTAAAGGAACTGAGAGAGAGAGTATTAAGAGGGAAATACAGAATTCCCTTCTACATGTCTACAGACTGT
  GAAAACCTTCTCAAACGTTTCCTGGTGCTAAATCCAATTAAACGCGGCACTCTAGAGCAAATCATGAAGG
  ACAGGTGGATCAATGCAGGGCATGAAGAAGATGAACTCAAACCATTTGTTGAACCAGAGCTAGACATCTC
  AGACCAAAAAAGAATAGATATTATGGTGGGAATGGGATATTCACAAGAAGAAATTCAAGAATCTCTTAGT
  AAGATGAAATACGATGAAATCACAGCTACATATTTGTTATTGGGGAGAAAATCTTCAGAGCTGGATGCTA
  GTGATTCCAGTTCTAGCAGCAATCTTTCACTTGCTAAGGTTAGGCCGAGCAGTGATCTCAACAACAGTAC
  TGGCCAGTCTCCTCACCACAAAGTGCAGAGAAGTGTTTTTTCAAGCCAAAAGCAAAGACGCTACAGTGAC
  CATGCTGGACCAGCTATTCCTTCTGTTGTGGCGTATCCGAAAAGGAGTCAGACCAGCACTGCAGATAGTG
  ACCTCAAAGAAGATGGAATTTCCTCCCGGAAATCAAGTGGCAGTGCTGTTGGAGGAAAGGGAATTGCTCC
  AGCCAGTCCCATGCTTGGGAATGCAAGTAATCCTAATAAGGCGGATATTCCTGAACGCAAGAAAAGCTCC
  ACTGTCCCTAGTAGTAACACAGCATCTGGTGGAATGACACGACGAAATACTTATGTTTGCAGTGAGAGAA
  CTACAGCTGATAGACACTCAGTGATTCAGAATGGCAAAGAAAACAGCACTATTCCTGATCAGAGAACTCC
  AGTTGCTTCAACACACAGTATCAGTAGTGCAGCCACCCCAGATCGAATCCGCTTCCCAAGAGGCACTGCC
  AGTCGTAGCACTTTCCACGGCCAGCCCCGGGAACGGCGAACCGCAACATATAATGGCCCTCCTGCCTCTC
  CCAGCCTGTCCCATGAAGCCACACCATTGTCCCAGACTCGAAGCCGAGGCTCCACTAATCTCTTTAGTAA
  ATTAACTTCAAAACTCACAAGGAGTCGCAATGTATCTGCTGAGCAAAAAGATGAAAACAAAGAAGCAAAG
  CCTCGATCCCTACGCTTCACCTGGAGCATGAAAACCACTAGTTCAATGGATCCCGGGGACATGATGCGGG
  AAATCCGCAAAGTGTTGGACGCCAATAACTGCGACTATGAGCAGAGGGAGCGCTTCTTGCTCTTCTGCGT
  CCACGGAGATGGGCACGCGGAGAACCTCGTGCAGTGGGAAATGGAAGTGTGCAAGCTGCCAAGACTGTCT
  CTGAACGGGGTCCGGTTTAAGCGGATATCGGGGACATCCATAGCCTTCAAAAATATTGCTTCCAAAATTG
  CCAATGAGCTAAAGCTGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002376 unedited
  ACGCCCGTCGAGCAAAGGGCGGTAGGCGCTGTACGGTTGGGAGGTTCTATATAAGCAGAGCTCGTTTAGT
  GAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGGATCTAG
  ACGGCCGTAGGGGGAAGGGAGCCGCCCTCCCCACGGCGCCTTTTCGGAACTGCCGTGGACTCGAGGACGC
  TGGTCGCCGGCCTCCTAGGGCTGTGCTGTTTTGTTTTGACCCTCGCATTGTGCAGAATTAAAGTGCAGTA
  AAATGTCCACTAGGACCCCATTGCCAACGGTGAATGAACGAGACACTGAAAACCACACGTCACATGGAGA
  TGGGCGTCAAGAAGTTACCTCTCGTACCAGCCGCTCAGGAGCTCGGTGTAGAAACTCTATAGCCTCCTGT
  GCAGATGAACAACCTCACATCGGAACTACAGACTGTTGAAAACAATCGGCAAAGGGAATTTTGCAAAGTA
  AAATTGGCAAGACATATCCTTACAGGCAGAGAGGTTGCAATAATGATAATTGACAAAACTCAGTTGAATC
  CAACAAGTCTACAAAGCTCTTCAGAGAGTAGATATGAGATTTAAATCATCCCATATAGTGAAGTTATCGA
  GTCATTGAACTGAAAAAACACTCTACCTATCATGATATTGCAGTGGAGGTGGAGTTATTTGACTTTTTGT
  TGCACCATGCAGATGAGGAAAAGAAGCAGATTCTAATTAGAACGATTGTGGTCTTGCAGTCATACTGCAT
  CAGAACGATCGTAACATCGAGAACCTAGCTGAAATCTATTTGTTAGATGCGATTGAACATAAATAGCGGA
  TTTCCGGTTAGCATTGATTACTGTGGGCGGTAACTCGCACGGTTTTGGGCAGTCTTCTACGCAGCACCTT
  GGACCTTCAGGCAGAAATATGAACGGCCTATTGAT
  
  Kinase domain sequence

  >SC323372 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  AKCTGCAACATCGGCAGGGGATTTTGCAAAGTAAAATTGGCAAGACATATCCTTACAGGCAGAGAGGTTG
  CAATAATGATAATTGACAAAACTCAGTTGAATCCAACAAGTCTACAAAAGCTCTTCAGAGAAGTAAGAAT
  AATGAAGATTTTAAATCATCCCAATATAGTGAAGTTATTCGAAGTCATTGAAACTGAAAAAACACTCTAC
  CTAATCATGGAATATGCAAGTGGAGGTGAAGTATTTGACTATTTG
  
• Restriction Sites: Please inquire
• ACCN: NM_002376
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002376.4, NP_002367.4
• RefSeq Size: 3404 bp
• RefSeq ORF: 2190 bp
• Locus ID: 4140
• UniProt ID: P27448
• Cytogenetics: 14q32.32-q32.33
• Domains: UBA, pkinase, TyrKc, KA1, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene is activated by phosphorylation and in turn is involved in the phosphorylation of tau proteins MAP2 and MAP4. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
  Transcript Variant: This variant (3) lacks two alternate in-frame exons compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a.