DNA Polymerase beta (POLB) (NM_002690) Human Untagged Clone

CAT#: SC322059

POLB (untagged)-Human polymerase (DNA directed), beta (POLB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNA Polymerase beta
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322059 ACCATTGTTCCGCCGGTCGCGCCGGAGCTGGGTTGCTCCTGCTCCCGTCTCCAAGTCCTG
  GTACCTCCTTCAAGCTGGGAGAGGGCTCTAGTCCCTGGTTCTGAACACTCTGGGGTTCTC
  GGGTGCAGGCCGCCATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCA
  CCGACATGCTCACAGAACTCGCAAACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGT
  ACAATGCTTACAGAAAAGCAGCATCTGTTATAGCAAAATACCCACACAAAATAAAGAGTG
  GAGCTGAAGCTAAGAAATTGCCTGGAGTAGGAACAAAAATTGCTGAAAAGATTGATGAGT
  TTTTAGCAACTGGAAAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGAGTTCAT
  CCATCAATTTCCTGACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAG
  ATGAAGGAATTAAAACACTAGAAGATCTCAGAAAAAATGAAGATAAATTGAACCATCATC
  AGCGAATTGGGCTGAAATATTTTGGGGACTTTGAAAAAAGAATTCCTCGTGAAGAGATGT
  TACAAATGCAAGATATTGTACTAAATGAAGTTAAAAAAGTGGATTCTGAATACATTGCTA
  CAGTCTGTGGCAGTTTCAGAAGAGGTGCAGAGTCCAGTGGTGACATGGATGTTCTCCTGA
  CCCATCCCAGCTTCACTTCAGAATCAACCAAACAGCCAAAACTGTTACATCAGGTTGTGG
  AGCAGTTACAAAAGGTTCATTTTATCACAGATACCCTGTCAAAGGGTGAGACAAAGTTCA
  TGGGTGTTTGCCAGCTTCCCAGTAAAAATGATGAAAAAGAATATCCACACAGAAGAATTG
  ATATCAGGTTGATACCCAAAGATCAGTATTACTGTGGTGTTCTCTATTTCACTGGGAGTG
  ATATTTTCAATAAGAATATGAGGGCTCATGCCCTAGAAAAGGGTTTCACAATCAATGAGT
  ACACCATCCGTCCCTTGGGAGTCACTGGAGTTGCAGGAGAACCCCTGCCAGTGGATAGTG
  AAAAAGACATCTTTGATTACATCCAGTGGAAATACCGGGAACCCAAGGACCGGAGCGAAT
  GAGGCCTGTATCCTCCCTGGCAGACACAACCCAATAGGAGTCTTAATTTATTTCTTAACC
  TTTGCTATGTAAGGGTCTTTGGTGTTTTTAAATGATTGTTTCTTCTTCATGCTTTTGCTT
  GCAATGTAGTCAATAAAACCTCATGTACTATTAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002690
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002690.1, NP_002681.1
• RefSeq Size: 1259 bp
• RefSeq ORF: 1008 bp
• Locus ID: 5423
• UniProt ID: P06746
• Cytogenetics: 8p11.21
• Domains: POLXc, HhH1
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Gene Summary: The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]