HDAC2 (NM_001527) Human Untagged Clone

CAT#: SC317003

HDAC2 (untagged)-Human histone deacetylase 2 (HDAC2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HDAC2
• Synonyms: HD2; KDAC2; RPD3; YAF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001527 edited
  CAGCAGCAGCAGCAGCAGCAGCAGGAGGAGGAGCCCGGTGGCGGCGGTGGCCGGGGAGCC
  CATGGCGTACAGTCAAGGAGGCGGCAAAAAAAAAGTCTGCTACTACTACGACGGTGATAT
  TGGAAATTATTATTATGGACAGGGTCATCCCATGAAGCCTCATAGAATCCGCATGACCCA
  TAACTTGCTGTTAAATTATGGCTTATACAGAAAAATGGAAATATATAGGCCCCATAAAGC
  CACTGCCGAAGAAATGACAAAATATCACAGTGATGAGTATATCAAATTTCTACGGTCAAT
  AAGACCAGATAACATGTCTGAGTATAGTAAGCAGATGCAGAGATTTAATGTTGGAGAAGA
  TTGTCCAGTGTTTGATGGACTCTTTGAGTTTTGTCAGCTCTCAACTGGCGGTTCAGTTGC
  TGGAGCTGTGAAGTTAAACCGACAACAGACTGATATGGCTGTTAATTGGGCTGGAGGATT
  ACATCATGCTAAGAAATCAGAAGCATCAGGATTCTGTTACGTTAATGATATTGTGCTTGC
  CATCCTTGAATTACTAAAGTATCATCAGAGAGTCTTATATATTGATATAGATATTCATCA
  TGGTGATGGTGTTGAAGAAGCTTTTTATACAACAGATCGTGTAATGACGGTATCATTCCA
  TAAATATGGGGAATACTTTCCTGGCACAGGAGACTTGAGGGATATTGGTGCTGGAAAAGG
  CAAATACTATGCTGTCAATTTTCCAATGAGAGATGGTATAGATGATGAGTCATATGGGCA
  GATATTTAAGCCTATTATCTCAAAGGTGATGGAGATGTATCAACCTAGTGCTGTGGTATT
  ACAGTGTGGTGCAGACTCATTATCTGGTGATAGACTGGGTTGTTTCAATCTAACAGTCAA
  AGGTCATGCTAAATGTGTAGAAGTTGTAAAAACTTTTAACTTACCATTACTGATGCTTGG
  AGGAGGTGGCTACACAATCCGTAATGTTGCTCGATGTTGGACATATGAGACTGCAGTTGC
  CCTTGATTGTGAGATTCCCAATGAGTTGCCATATAATGATTACTTTGAGTATTTTGGACC
  AGACTTCAAACTGCATATTAGTCCTTCAAACATGACAAACCAGAACACTCCAGAATATAT
  GGAAAAGATAAAACAGCGTTTGTTTGAAAATTTGCGCATGTTACCTCATGCACCTGGTGT
  CCAGATGCAAGCTATTCCAGAAGATGCTGTTCATGAAGACAGTGGAGATGAAGATGGAGA
  AGATCCAGACAAGAGAATTTCTATTCGAGCATCAGACAAGCGGATAGCTTGTGATGAAGA
  ATTCTCAGATTCTGAGGATGAAGGAGAAGGAGGTCGAAGAAATGTGGCTGATCATAAGAA
  AGGAGCAAAGAAAGCTAGAATTGAAGAAGATAAGAAAGAAACAGAGGACAAAAAAACAGA
  CGTTAAGGAAGAAGATAAATCCAAGGACAACAGTGGTGAAAAAACAGATACCAAAGGAAC
  CAAATCAGAACAGCTCAGCAACCCCTGAATTTGACAGTCTCACCAATTTCAGAAAATCAT
  TAAAAAGAAAATATTGAAAGGAAAATGTTTTCTTTTTGAAGACTTCTGGCTTCATTTTAT
  ACTACTTTGGCATGGACTGTATTTATTTTCAAATGGCTTTTTCGTTTTTGTTTTTCTTGG
  CAAGTTTTATTGTGAGTTTTTCTAATTATGAAGCAAAATTTCTTTTCTCCACCATGCTTT
  ATGTGATAGTATTTAAAATTGATGTGAGTTATTATGTCAAAAAAACTGATCTATTAAAGA
  AGTAATTGGCCTTTCTGAGCTGATTTTTCCATCTTTTGTAATTATCTTTATTAAAAAATT
  GTACTTGGAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001527 unedited
  TACCTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGGCACGAGGATGCGCTCACCTC
  CCTGCGGCCTCCTGAGGTGGTTTGGTGGCCCCCTCCTCGCGAGTTGGTGCCGCTGCCACC
  TCCGATTCCGAGCTTTCGGCACCTCTGCCGGGTGGTACCGAGCCTTCCCGGCGCCCCCTC
  CTCTCCTCCCACCGGCCTGCCCTTCCCCGCGGGACTATCGCCCCCACGTTTCCCTCAGCC
  CTTTTCTCTCCCGGCCGAGCCGCGGCGGCAGCAGCAGCAGCAGCAGCAGCAGCAGGAGGA
  GGAGCCCGGTGGCGGCGGTGGCCGGGGAGCCCATGGCGTACAGTCAAGGAGGCGGCAAAA
  AAAAAGTCTGCTACTACTACGACGGTGATATTGGAAATTATTATTATGGACAGGGTCATC
  CCATGAAGCCTCATAGAATCCGCATGACCCATAACTTGCTGTTAAATTATGGCTTATACA
  GAAAAATGGAAATATATAGGCCCCATAAAGCCACTGCCGAAGAAATGACAAAATATCACA
  GTGATGAGTATATCAAATTTCTACGGTCAATAAGACCAGATAACATGTCTGAGTATAGTA
  AGCAGATGCAGAGATTTAATGTTGGAGAAGATTGTCCAGTGTTTGATGGACTCTTTGAGT
  TTTGTCAGCTCTCAACTGGCGGTTCAGTTGCTGGAGCTGTGAAGTTAAACCCGACAACAG
  ACTGATATGGCTTGTTAATTTGGCTGGAGATTACATCATGCTAAGAAATCAGAGCATCAG
  ATTCTGTTACGTAATGATATTTGTGCTGCATCTGATACTAAGTATCATCAGAAGAGTCTA
  TATATTGAATATAGATATCATCATGTGATTGTGTTGAGAGCTTTTTATACAAACAGATCG
  GTGTATGACGTATTCATTCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001527 unedited
  CGAGATGCACTTCAGGGCCGGAAGCACCGGGTGAGGGGTCACAGGGATGCCACCCGGGAT
  CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTT
  TTTTCCAAGTACAATTTTTTAATAAAGATAATTACAAAAGATGGAAAAATCAGCTCAAAA
  AGGCCAATTACTTCTTTAATAGATCAGTTTTTTTGACATAATAACTCACATCAATTTTAA
  ATACTATCACATAAAGCATGGTGGAAAAAAAAAATTTTGCTTCATAATTAGAAAAACTCA
  CAATAAAACTTGCCAAGAAAAACAAAAACGAAAAAGCCATTTGAAAATAAATACAGTCCA
  TGCCAAAGTAGTATAAAATGAAGCCAGAAGTCTTCAAAAAGAAAACATTTTCCTTTCAAT
  ATTTTCTTTTTAATGATTTTCTGAAATTGGTGAGACTGTCAAATTCAGGGGTTGCTGAGC
  TGTTCTGATTTGGTTCCTTTGGTATCTGTTTTTTCACCACTGTTGTCCTTGGATTTATCT
  TCTTCCTTAACGTCTGTTTTTTTGTCCTCTGTTTCTTTCTTATCTTCTTCAATTCTAGCT
  TTCTTTGCTCCTTTCTTATGATCAGCCACATTTCTTCGACCTCCTTCTCCTTCATCCTCA
  GAATCTGAGAATTCTTCATCACAAGCTATCCGCTTGTCTGATGCTCGAATAGAAATTCTC
  TTGTCTGGATCTTCTCCATCTTCATCTCCACTGTCTTCATGACAGCATCTTCTGGAATAG
  CTTGCATCTGGACACAGTGCATGAGTACATGCGCAATTTTCAAACAACGCTGTTTATCTT
  TCATATATTCTGGAGTGTCTGTTGTCATGTGAGACTATATGCAGTGAGTCGTCCAATACT
  CAGTATCATATGCACTCATGGCATCTCCATCAGGCACTGCG
  
• Restriction Sites: Please inquire
• ACCN: NM_001527
• Insert Size: 1900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001527.2, NP_001518.2
• RefSeq Size: 6659 bp
• RefSeq ORF: 1749 bp
• Locus ID: 3066
• UniProt ID: Q92769
• Cytogenetics: 6q21
• Domains: Hist_deacetyl
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, Chronic myeloid leukemia, Huntington's disease, Notch signaling pathway, Pathways in cancer
• Gene Summary: This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010]
  Transcript Variant: This variant (1) encodes the functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.